Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.
Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.
Int J Biol Macromol. 2014 Mar;64:84-9. doi: 10.1016/j.ijbiomac.2013.11.031. Epub 2013 Dec 4.
The present study aimed to investigate and evaluate the efficiency of immobilizing the Lactobacillus plantarum TN9 strain in alginate using chitosan and gelatin as coating materials, in terms of viability and antibacterial activity. The results indicate that maximum concentrations of L. plantarum TN9 strain were produced with 2% sodium alginate, 10(8)UFC/ml, and 1M calcium chloride. The viability and antibacterial activity of the L. plantarum TN9 cultures before and after immobilization in alginate, chitosan-coated alginate, and gelatin-coated alginate, were studied. The findings revealed that the viability of encapsulated L. plantarum could be preserved more than 5.8 log CFU/ml after 35 day of incubation at 4 °C, and no effects were observed when gelatin was used. The antibacterial activity of encapsulated L. plantarum TN9 against Gram-positive and Gram-negative pathogenic bacteria was enhanced in the presence of chitosan coating materials, and no activity was observed in the presence of gelatin. The effects of catalase and proteolytic enzymes on the culture supernatant of L. plantarum TN9 were also investigated, and the results suggested that the antibacterial activity observed was due to the production of organic acids. Taken together, the findings indicated that immobilization in chitosan enhanced the antibacterial activity of L. plantarum TN9 against several pathogenic bacteria. This encapsulated strain could be considered as a potential strong candidate for future application as an additive in the food and animal feed industries.
本研究旨在探讨和评估使用壳聚糖和明胶作为包埋材料将植物乳杆菌 TN9 菌株固定在海藻酸钠中的效率，主要从存活率和抗菌活性两方面进行评估。结果表明，当使用 2%的海藻酸钠、10(8)UFC/ml 和 1M 氯化钙时，植物乳杆菌 TN9 菌株的最大浓度最高。本研究还对固定在海藻酸钠、壳聚糖包被的海藻酸钠和明胶包被的海藻酸钠中的植物乳杆菌 TN9 培养物的存活率和抗菌活性进行了研究。研究发现，在 4°C 下孵育 35 天后，包埋的植物乳杆菌的存活率可保持在 5.8 个对数 CFU/ml 以上，而使用明胶时则没有观察到效果。当存在壳聚糖包埋材料时，包埋的植物乳杆菌 TN9 对革兰氏阳性和革兰氏阴性致病菌的抗菌活性增强，而当存在明胶时则没有观察到活性。本研究还研究了过氧化氢酶和蛋白水解酶对植物乳杆菌 TN9 培养上清液的影响，结果表明观察到的抗菌活性是由于有机酸的产生。总的来说，研究结果表明，壳聚糖的固定增强了植物乳杆菌 TN9 对几种致病菌的抗菌活性。这种包埋菌株可以被认为是未来在食品和动物饲料工业中作为添加剂应用的潜在有力候选菌株。
