Faculty of Chemical Engineering and Technology, Cracow University of Technology, 31-155 Cracow, Poland.
Lukasiewicz Research Network─Institute of Industrial Organic Chemistry, 03-236 Warsaw, Poland.
ACS Appl Mater Interfaces. 2024 Oct 2;16(39):52878-52893. doi: 10.1021/acsami.4c10187. Epub 2024 Sep 20.
To realize the health benefits of probiotic bacteria, they must withstand processing and storage conditions and remain viable after use. The encapsulation of these probiotics in the form of microspheres containing tapioca flour as a prebiotic and vehicle component in their structure or shell affords symbiotic effects that improve the survival of probiotics under unfavorable conditions. Microencapsulation is one such method that has proven to be effective in protecting probiotics from adverse conditions while maintaining their viability and functionality. The aim of the work was to obtain high-quality microspheres that can act as carriers of bacteria and to assess the impact of encapsulation on the viability of probiotic microorganisms in alginate microspheres enriched with a prebiotic (tapioca flour) and additionally coated with hyaluronic acid, chitosan, or gelatin. The influence of the composition of microparticles on the physicochemical properties and the viability of probiotic bacteria during storage was examined. The optimal composition of microspheres was selected using the design of experiments using statistical methods. Subsequently, the size, morphology, and cross-section of the obtained microspheres, as well as the effectiveness of the microsphere coating with biopolymers, were analyzed. The chemical structure of the microspheres was identified by using Fourier-transform infrared spectrophotometry. Raman spectroscopy was used to confirm the success of coating the microspheres with the selected biopolymers. The obtained results showed that the addition of tapioca flour had a positive effect on the surface modification of the microspheres, causing the porous structure of the alginate microparticles to become smaller and more sealed. Moreover, the addition of prebiotic and biopolymer coatings of the microspheres, particularly using hyaluronic acid and chitosan, significantly improved the survival and viability of the probiotic strain during long-term storage. The highest survival rate of the probiotic strain was recorded for alginate-tapioca flour microspheres coated with hyaluronic acid, at 5.48 log CFU g. The survival rate of in that vehicle system was 89% after storage for 30 days of storage.
为了实现益生菌的健康益处，它们必须能够耐受加工和储存条件，并在使用后保持存活。通过将这些益生菌封装在含有木薯淀粉的微球形式中，作为结构或壳中的前体和载体成分，可以获得共生效应，从而改善益生菌在不利条件下的生存能力。微囊化就是这样一种方法，已被证明可以有效地保护益生菌免受不利条件的影响，同时保持其存活和功能。本工作的目的是获得高质量的微球，作为细菌的载体，并评估封装对富含前体（木薯淀粉）的海藻酸钠微球中益生菌微生物存活能力的影响，此外还可以用透明质酸、壳聚糖或明胶对其进行涂层。考察了微颗粒的组成对益生菌在储存过程中的物理化学性质和存活能力的影响。使用统计方法的实验设计选择了最佳的微球组成。随后，分析了所获得的微球的大小、形态和横截面，以及生物聚合物微球涂层的有效性。通过傅里叶变换红外光谱法对微球的化学结构进行了鉴定。拉曼光谱法用于确认所选生物聚合物成功地对微球进行了涂层。研究结果表明，木薯淀粉的添加对微球的表面改性有积极影响，使海藻酸钠微球的多孔结构变小且更加封闭。此外，添加前体和生物聚合物涂层，特别是使用透明质酸和壳聚糖，显著提高了益生菌菌株在长期储存过程中的存活率和活力。在含有透明质酸的海藻酸钠-木薯淀粉微球中，益生菌菌株的存活率最高，为 5.48logCFUg。在该载体系统中，经过 30 天的储存后，存活率为 89%。
