Gómez-Camarasa Cristina, Gutiérrez-Fernández José, Rodríguez-Granger Javier Moises, Sampedro-Martínez Antonio, Sorlózano-Puerto Antonio, Navarro-Marí José María
Laboratorio de Microbiología, Hospital Universitario Virgen de las Nieves, Spain.
Laboratorio de Microbiología, Hospital Universitario Virgen de las Nieves, Spain; Departamento de Microbiología, Universidad de Granada, 18014 Granada, Spain.
Diagn Microbiol Infect Dis. 2014 Feb;78(2):101-4. doi: 10.1016/j.diagmicrobio.2013.11.009. Epub 2013 Nov 15.
The study objective was to evaluate the effectiveness of the new rapid immunochromatographic test RIDAQUICK Campylobacter® (r-biopharm AG, Darmstadt, Germany) for the qualitative detection of Campylobacter antigens in pathologic feces from primary and specialist care patients. Three hundred feces samples were studied from patients with diarrhea, 50.6% from adults and 49.4% from children, which were received by our microbiology laboratory for coproculture. Campylobacter culture results, with or without PCR data, served as reference values for the comparative evaluation of RIDAQUICK Campylobacter® findings. Campylobacter was detected in 12.3% of samples. The diagnostic accuracy values of the RidaQuick Campylobacter® versus culture were: sensitivity of 87%, specificity of 97%, and positive and negative predictive values of 77% and 98%, respectively. RIDAQUICK Campylobacter® is a rapid test for the diagnosis of enteritis due to Campylobacter and could be an option for the clinical diagnosis of one of the main causes of bacterial enteritis in resource-limited settings.
本研究的目的是评估新型快速免疫层析检测试剂RIDAQUICK Campylobacter®（r-生物制药股份公司，德国达姆施塔特）用于定性检测基层医疗和专科医疗患者病理粪便中弯曲杆菌抗原的有效性。对300份腹泻患者的粪便样本进行了研究，其中50.6%来自成人，49.4%来自儿童，这些样本由我们的微生物实验室接收用于粪便培养。弯曲杆菌培养结果（无论有无PCR数据）作为RIDAQUICK Campylobacter®检测结果比较评估的参考值。12.3%的样本检测出弯曲杆菌。RidaQuick Campylobacter®相对于培养的诊断准确性数值为：灵敏度87%，特异性97%，阳性预测值和阴性预测值分别为77%和98%。RIDAQUICK Campylobacter®是一种用于诊断弯曲杆菌所致肠炎的快速检测试剂，在资源有限的环境中，它可能是临床诊断细菌性肠炎主要病因之一的一种选择。
