Özcan Nida, Bacalan Fatma, Çakır Fatih, Bilden Alican, Genişel Neslihan, Dal Tuba
Dicle University Medicine Faculty, Medical Microbiology Department, Diyarbakır, Turkey.
Diyarbakır Children's Hospital, Laboratory of Microbiology, Diyarbakır, Turkey.
J Infect Dev Ctries. 2022 Apr 30;16(4):616-621. doi: 10.3855/jidc.14902.
Campylobacter infections are among the most common causes of bacterial enteritis. This study aims to determine the sensitivity, specificity and positive predictive values (PPV) of culture and culture-independent tests for the diagnosis of Campylobacter enteritis.
A total of 400 stool samples were included in the study. BD MAX enteric bacterial panel (BD Diagnostics, Franklin Lakes, NJ, USA) and EntericBio Gastro Panel II (Serosep, Limerick, Ireland) were used as commercial molecular tests. RIDA®QUICK Campylobacter (R-Biopharm, Darmstadt,, Germany) and CerTest (Biotec, Zaragoza, Spain) were used to detect Campylobacter antigens. Samples were cultured in CCDA media and subjected to bacterial identification by mass spectrometry.
Among the 400 specimens, 41 (10.2%) were evaluated as Campylobacter positive; 21 were culture-positive and 20 were detected as positive by both PCR methods. Of the 21 isolates grown in culture, 16 (76.2%) were identified as C. jejuni and 5 (23.8%) as C. coli. While all 21 culture-positive specimens were detected as positive by both molecular tests, 18 of the specimens were found positive by RidaQuick, and 16 by Certest ICA. Of the 20 culture-negative Campylobacter cases, 18 were positive by RidaQuick and 12 by Certest ICA. Sensitivities of culture, ICA-RidaQuick and ICA-CerTest were 51.2%, 87.8 and 68.3, respectively. The specificities of all tests were in the range of 90-100 %. PPV of molecular tests, ICA-RidaQuick and ICA-CerTest were > 95%, 72 % and 48.3 %, respectively.
Molecular tests were superior to culture and ICA in terms of sensitivity, specificity, and positive predictive value.
弯曲杆菌感染是细菌性肠炎最常见的病因之一。本研究旨在确定用于诊断弯曲杆菌肠炎的培养法和非培养检测方法的敏感性、特异性和阳性预测值（PPV）。
本研究共纳入400份粪便样本。BD MAX肠道细菌检测板（BD诊断公司，美国新泽西州富兰克林湖）和EntericBio胃肠检测板II（Serosep公司，爱尔兰利默里克）用作商业分子检测方法。RIDA®QUICK弯曲杆菌检测试剂盒（R - Biopharm公司，德国达姆施塔特）和CerTest检测试剂盒（Biotec公司，西班牙萨拉戈萨）用于检测弯曲杆菌抗原。样本在CCDA培养基中培养，并通过质谱进行细菌鉴定。
在400份标本中，41份（10.2%）被评估为弯曲杆菌阳性；21份培养阳性，20份通过两种PCR方法均检测为阳性。在培养出的21株分离菌中，16株（76.2%）被鉴定为空肠弯曲菌，5株（23.8%）为大肠弯曲菌。虽然所有21份培养阳性标本通过两种分子检测方法均检测为阳性，但其中18份标本通过RidaQuick检测为阳性，16份通过Certest ICA检测为阳性。在20份培养阴性的弯曲杆菌病例中，18份通过RidaQuick检测为阳性，12份通过Certest ICA检测为阳性。培养法、ICA - RidaQuick和ICA - CerTest的敏感性分别为51.2%、87.8%和68.3%。所有检测方法的特异性在90% - 100%范围内。分子检测方法、ICA - RidaQuick和ICA - CerTest的PPV分别> 95%、72%和48.3%。
分子检测方法在敏感性、特异性和阳性预测值方面优于培养法和免疫捕获法。
