Liébana-Martos Ma del Carmen, Gutierrez José, Riazzo Cristina, Navarro José Ma
Mª del Carmen Liébana Martos, Servicio de Microbiología. Hospital Universitario Virgen de las Nieves. Avda. Fuerzas Armadas, nº 2. 18014, Granada, Spain.
Rev Esp Quimioter. 2014 Jun;27(2):102-5.
Introduction: Campylobacter sp. and Salmonella enterica are two of the main organisms causing gastroenteritis in our environment. Immunochromatographic tests for antigen detection performed directly on stool samples for its simplicity and rapid results may make them useful diagnostic elements in the context of primary care.
During October 2012 we selected all feces in which enteropathogenic bacteria are isolated from those received for stool culture in the laboratory of Microbiology of the University Hospital Virgen de las Nieves of Granada. After standard management of faeces samples and isolation of any enteropathogen, the commercial kits: Campy Leti, Ridaquick Campylobacterscreen and Salmonella Leti were tested for simultaneous research of Campylobacter and Salmonella antigens. Sensitivity and specificity were determined.
Two hundred and thirty five stool samples were received in which 8 Salmonella enterica (7 B serogroup and 1 D serogroup), 7 Campylobacter jejuni, 4 Aeromonas hydrophila and 1 Yersinia enterocolitica were isolated. Campy Leti, Ridaquick Campylobacterscreen and Salmonella Leti presented a sensitivity of 100%, 100% and 75%, respectively. Specificities corresponded to 46%, 69% and 100%, respectively.
Immunocromatographic tests can be useful for a first screening of enteropathogen in primary care.
引言：弯曲杆菌属和肠炎沙门氏菌是我们环境中引起肠胃炎的两种主要病原体。直接在粪便样本上进行抗原检测的免疫层析试验因其简单性和快速出结果，可能使其成为初级保健背景下有用的诊断要素。
2012年10月，我们从格拉纳达维尔根德拉斯涅韦斯大学医院微生物学实验室接收的粪便培养样本中，挑选出所有分离出肠道致病菌的粪便。在对粪便样本进行标准处理并分离出任何肠道病原体后，对商业试剂盒Campy Leti、Ridaquick Campylobacterscreen和Salmonella Leti进行了弯曲杆菌和沙门氏菌抗原的同步检测。测定了敏感性和特异性。
共收到235份粪便样本，其中分离出8株肠炎沙门氏菌（7株B血清群和1株D血清群）、7株空肠弯曲杆菌、4株嗜水气单胞菌和1株小肠结肠炎耶尔森菌。Campy Leti、Ridaquick Campylobacterscreen和Salmonella Leti的敏感性分别为100%、100%和75%。特异性分别为46%、69%和100%。
免疫层析试验可用于初级保健中肠道病原体的初步筛查。
