He X, Zhang X-M, Wu J, Fu J, Mou L, Lu D-H, Cai Y, Luo X-G, Pan A, Yan X-X
Department of Anatomy and Neurobiology, Central South University Xiangya School of Medicine, Changsha, Hunan 410013, China.
Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
Neuroscience. 2014 Feb 14;259:101-12. doi: 10.1016/j.neuroscience.2013.11.056. Epub 2013 Dec 4.
Immature neurons expressing doublecortin (DCX+) are present around cortical layer II in various mammals including guinea pigs and humans, especially enriched in the paleocortex. However, little is known whether and how functional experience affects the development of this population of neurons. We attempted to explore a modulation by experience to layer II DCX+ cells in the primary olfactory cortex in postnatal and adult guinea pigs. Neonatal and 1-year-old guinea pigs were subjected to unilateral naris-occlusion, followed 1 and 2months later by morphometry of DCX+ cells in the piriform cortex. DCX+ somata and processes were reduced in the deprived relative to the non-deprived piriform cortex in both age groups at the two surviving time points. The number of DCX+ cells was decreased in the deprived side relative to internal control at 1 and 2months in the youths and at 2months in the adults post-occlusion. The mean somal area of DCX+ cells showed a trend of decrease in the deprived side relative to the internal control in the youths. In addition, DCX+ cells in the deprived side exhibited a lower frequency of colocalization with the neuron-specific nuclear antigen (NeuN) relative to counterparts. These results suggest that normal olfactory experience is required for the maintenance and development of DCX+ immature neurons in postnatal and adult guinea pig piriform cortex.
在包括豚鼠和人类在内的各种哺乳动物中，表达双皮质素（DCX+）的未成熟神经元存在于皮质层II周围，尤其在古皮质中富集。然而，关于功能体验是否以及如何影响这群神经元的发育，我们知之甚少。我们试图探究出生后和成年豚鼠初级嗅觉皮质中，体验对II层DCX+细胞的调节作用。对新生和1岁的豚鼠进行单侧鼻孔闭塞，在1个月和2个月后，对梨状皮质中的DCX+细胞进行形态测量。在两个存活时间点，两个年龄组中，与未剥夺的梨状皮质相比，剥夺侧的DCX+体细胞和突起均减少。在闭塞后的1个月和2个月，青年组以及成年组在2个月时，剥夺侧的DCX+细胞数量相对于内部对照减少。青年组中，相对于内部对照，剥夺侧的DCX+细胞平均体细胞面积呈减少趋势。此外，相对于对侧，剥夺侧的DCX+细胞与神经元特异性核抗原（NeuN）共定位的频率较低。这些结果表明，正常的嗅觉体验对于出生后和成年豚鼠梨状皮质中DCX+未成熟神经元的维持和发育是必需的。
