Department of Polymer Science and Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
Department of Polymer Science and Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
Colloids Surf B Biointerfaces. 2014 Mar 1;115:37-45. doi: 10.1016/j.colsurfb.2013.11.022. Epub 2013 Nov 21.
This study evaluated graphene oxide functionalized covalently with poly N-vinyl caprolactam (GO-PVCL) via in situ atomic transfer radical polymerization (ATRP), as a nano-cargo carrier for the efficient delivery of drugs into cells. Water-soluble GO-PVCL exhibited excellent stability in physiological solutions. An anti-cancer drug, camptothecin (CPT), was then loaded onto GO-PVCL with a high payload (20%) through π-π stacking and hydrophobic interactions, and its release could be controlled by varying the pH. PVCL grafted onto GO offers an additional advantage of targeted delivery according to temperature. GO-PVCL showed no obvious toxicity, whereas the CPT-loaded GO-PVCL showed high potency in killing cancer cells in vitro. The drug transportation mechanism was found to be energy-dependent endocytosis. Overall, this study revealed GO-PVCL to be a promising drug delivery vector with high biocompatibility, solubility and stability in physiological solutions, and good payload capacity owing to its small size, low cost, large specific area, ready scalability, and useful non-covalent interactions. This material is expected to be a novel material propitious for biomedical applications.
本研究通过原位原子转移自由基聚合（ATRP）将氧化石墨烯与聚 N-乙烯基己内酰胺（GO-PVCL）共价键合，评估了其作为将药物有效递送入细胞的纳米载药载体的性能。水溶性 GO-PVCL 在生理溶液中表现出优异的稳定性。然后，通过 π-π 堆积和疏水相互作用，将抗癌药物喜树碱（CPT）以高载药量（20%）负载到 GO-PVCL 上，通过改变 pH 值可以控制其释放。接枝在 GO 上的 PVCL 根据温度提供了靶向递送的额外优势。GO-PVCL 显示出没有明显的毒性，而负载 CPT 的 GO-PVCL 在体外杀死癌细胞方面表现出很高的效力。药物运输机制被发现是能量依赖性的内吞作用。总的来说，本研究表明 GO-PVCL 是一种很有前途的药物输送载体，具有高生物相容性、在生理溶液中的溶解度和稳定性，以及由于其小尺寸、低成本、大比表面积、易于规模化和有用的非共价相互作用而具有良好的载药量。这种材料有望成为一种有利于生物医学应用的新型材料。
