Estevão Lígia Reis Moura, Medeiros Juliana Pinto de, Baratella-Evêncio Liriane, Simões Ricardo Santos, Mendonça Fábio de Souza, Evêncio-Neto Joaquim
Acta Cir Bras. 2013 Dec;28(12):863-9. doi: 10.1590/s0102-86502013001200009.
To evaluate the effects of copaiba oil ointment (Copaifera langsdorffii) on dorsal skin flaps in rats.
Adult male rats (n=30) were distributed into three groups of ten animals each, as follows: GC--control; GCA--absolute control and GT--treated with copaiba ointment. The rats were subjected to dorsal cutaneous skin flap surgery and the animals from the GC and GT received post-operative treatment for eight consecutive days. The animals from the GCA group did not receive treatment while the animals from the GC group received daily topical treatment of ointment without the active ingredient and the animals from the GT group were daily treated with 10% copaiba oil ointment. At the end of each experimental period the lesions were evaluated according to the percentage of necrotic area. Then, fragments from cranial, median and caudal parts were fixed in Boüin's solution and processed for paraffin embedding. The morphology of histological sections (5µm) was evaluated and the number of leucocytes, fibroblasts and blood vessels was also analyzed. The data obtained were submitted to ANOVA test complemented by Tukey-Kramer test (p<0.05).
The necrotic area was lower in the group treated with copaiba ointment when compared to the control groups (GCA>GC and GT), while the morphology showed larger granulation tissue with bulky fibroblasts and collagen fibers more arranged in the GT group. The morphometry showed a significant higher number of blood vessels in the median and caudal parts (GT>GCA and GC), leucocytes in the cranial part (GT>GC>GCA), and also fibroblasts in the median (GT and GC> GCA) and caudal parts (GT>GC and GCA) (p<0.05).
The copaiba oil ointment favors angiogenesis and accelerates the viability of random skin flaps in rats.
评估巴西香脂油软膏（Copaifera langsdorffii）对大鼠背部皮瓣的影响。
成年雄性大鼠（n = 30）被分为三组，每组十只动物，如下：GC组——对照组；GCA组——绝对对照组；GT组——用巴西香脂软膏治疗组。对大鼠进行背部皮肤皮瓣手术，GC组和GT组的动物连续八天接受术后治疗。GCA组的动物不接受治疗，GC组的动物每天接受不含活性成分的软膏局部治疗，GT组的动物每天用10%的巴西香脂油软膏治疗。在每个实验期结束时，根据坏死面积百分比评估损伤情况。然后，将取自头部、中部和尾部的组织碎片固定在波因氏液中，并进行石蜡包埋处理。评估组织学切片（5μm）的形态，并分析白细胞、成纤维细胞和血管的数量。所获得的数据进行方差分析，并辅以Tukey-Kramer检验（p<0.05）。
与对照组（GCA>GC和GT）相比，用巴西香脂软膏治疗的组坏死面积更低，而形态学显示GT组有更大的肉芽组织，有成纤维细胞体积较大且胶原纤维排列更有序。形态计量学显示，中部和尾部的血管数量显著更多（GT>GCA和GC），头部的白细胞数量更多（GT>GC>GCA），中部（GT和GC>GCA）和尾部（GT>GC和GCA）的成纤维细胞数量也更多（p<0.05）。
巴西香脂油软膏有利于血管生成，并加速大鼠随意皮瓣的存活。
