甘草酸二钾对伤口愈合的影响。
Effects of dipotassium glycyrrhizinate on wound healing.
作者信息
Leite Camila Dos Santos, Pires Oscar César, Tenis Danielle Gatti, Ziegler Jussara Vaz Nascimento, Priolli Denise Gonçalves, Rocha Thalita
机构信息
MSc, Fellow PhD degree. Multidisciplinary Research Laboratory, Universidade São Francisco (USF) - Bragança Paulista (SP), Brazil.
PhD, Assistant Professor. Physiology and Pharmacology Laboratory - Universidade de Taubaté (UNITAU) - Taubate (SP), Brazil.
出版信息
Acta Cir Bras. 2021 Oct 8;36(8):e360801. doi: 10.1590/ACB360801. eCollection 2021.
PURPOSE
Dipotassium glycyrrhizinate (DPG) has anti-inflammatory properties, besides promoting the regeneration of skeletal muscle. However, it has not been reported on skin wound healing/regeneration. This research aimed to characterize the effects of DPG in the treatment of excisional wounds by second intention.
METHODS
Male adults (n=10) and elderly (n=10) Wistar rats were used. Two circular wounds were excised on the dorsal skin. The excised normal skins were considered adult (GAN) and elderly (GIN) naïve. For seven days, 2% DPG was applied on the proximal excision: treated adult (GADPG) and elderly (GIDPG), whereas distal excisions were untreated adult (GANT) and elderly (GINT). Wound healing areas were daily measured and removed for morphological analyses after the 14th and the 21st postoperative day. Slides were stained with hematoxylin-eosin, Masson's trichrome, and picrosirius red.
RESULTS
Histological analysis revealed intact (GAN/GIN) and regenerated(GANT/GINT/GADPG/GIDPG) skins. No differences of wounds' size were found among treated groups. Epidermis was thicker after 14 days and thinner after 21 days of DPG administration. Higher collagen I density was found in GIDPG (14th day) and GADPG (21st day).
CONCLUSIONS
DPG induced woundhealing/skin regeneration, with collagen I, being more effective in the first 14 days after injury.
目的
甘草酸二钾（DPG）除了能促进骨骼肌再生外，还具有抗炎特性。然而，其对皮肤伤口愈合/再生的作用尚未见报道。本研究旨在探讨DPG对二期切除伤口治疗效果的特征。
方法
选用成年雄性（n = 10）和老年雄性（n = 10）Wistar大鼠。在背部皮肤切除两个圆形伤口。切除的正常皮肤分别作为成年未处理组（GAN）和老年未处理组（GIN）。连续7天，在近端切除伤口处涂抹2%的DPG：成年处理组（GADPG）和老年处理组（GIDPG），而远端切除伤口不做处理，分别作为成年未处理组（GANT）和老年未处理组（GINT）。每天测量伤口愈合面积，并在术后第14天和第21天切除伤口进行形态学分析。玻片用苏木精-伊红、Masson三色染色法和天狼星红染色。
结果
组织学分析显示皮肤完整（GAN/GIN）和再生（GANT/GINT/GADPG/GIDPG）。各处理组伤口大小无差异。DPG给药14天后表皮增厚，21天后变薄。GIDPG组（第14天）和GADPG组（第21天）的I型胶原蛋白密度更高。
结论
DPG可促进伤口愈合/皮肤再生，I型胶原蛋白起作用，在损伤后的前14天效果更明显。
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