Kitazaki Chifumi, Numano Setsuko, Takanezawa Akira, Nishizawa Tomoyasu, Shirai Makoto, Asayama Munehiko
College of Agriculture, Ibaraki University.
Biosci Biotechnol Biochem. 2013;77(12):2339-47. doi: 10.1271/bbb.130409. Epub 2013 Dec 7.
The cyanobacterium semi-filamentous multicellular strain ABRG5-3 undergoes cell lysis as a unique feature that occurs due to growth condition changes from normal cultivation with shaking to static cultivation without shaking in liquid culture (Nishizawa et al., 2010). Microscopic observation and energy dispersive X-ray spectrometer (EDX) analysis have revealed that lysis is involved in the accumulation of polyphosphate compounds and the disintegration of thylakoid membranes in cells. Static cultivation, dark or red light exposure, and temperature (22 to 42 °C) conditions were found to be effective factors for the induction of lysis. Moreover, stress induced by salts, osmotic pressure with sucrose, and the depletion of nitrogen or phosphate in cultures also induced ABRG5-3 cell lysis. Based on these results, we discuss lysis and its utilization in the biotechnology industry.
蓝藻半丝状多细胞菌株ABRG5-3会发生细胞裂解，这是一种独特的现象，它是由于液体培养中生长条件从正常振荡培养转变为无振荡静置培养而产生的（Nishizawa等人，2010年）。显微镜观察和能量色散X射线光谱仪（EDX）分析表明，裂解与细胞中多磷酸盐化合物的积累以及类囊体膜的解体有关。发现静置培养、黑暗或红光照射以及温度（22至42°C）条件是诱导裂解的有效因素。此外，盐诱导的应激、蔗糖引起的渗透压以及培养物中氮或磷的耗尽也会诱导ABRG5-3细胞裂解。基于这些结果，我们讨论了裂解及其在生物技术产业中的应用。
