Trencart P, Elce Y A, Rodriguez Batista E, Michaud G
Yvonne A. Elce, DVM DACVS, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Montreal, St Hyacinthe, Quebec, J2S 7C6, Canada, Phone: +1 450 778 8100, E-mail:
Vet Comp Orthop Traumatol. 2014;27(2):97-101. doi: 10.3415/VCOT-13-03-0037. Epub 2013 Dec 9.
Ethylene oxide is currently recommended for sterilization of antibiotic impregnated beads; however this method carries health risks to personnel and is becoming less available.
To perform a pilot study of the effect of radiation for sterilization of polymethylmethacrylate (PMMA) and plaster of Paris (POP) beads impregnated with amikacin, enrofloxacin, and ceftiofur.
Radiation would effectively sterilize the beads without affecting the efficacy of the antibiotic.
Beads of PMMA and POP were prepared in a clean but non-sterile manner with one of the three antibiotics (amikacin, enrofloxacin, ceftiofur) or no antibiotic. Beads were then exposed to radiation for a total dose of 0 kiloGray (kGy), 10 kGy and 25 kGy. Beads were incubated on Mueller-Hinton agar plates seeded with Escherichia coli, Staphylococcus aureus or Pseudomonas aeruginosa for 24 hours or cultured in brain-heart infusion broth for 48 hours. Zones of inhibition were measured on the agar plates and statistics were performed on the diameters of the zones of inhibition using an analysis of variance.
There were no differences in the diameters of inhibition for all levels of radiation for all PMMA beads. The same was true with POP beads with the exception of enrofloxacin which had a significantly decreased zone of inhibition with increased levels of radiation, though the clinical significance of this finding was not assessed. Only beads without antibiotics and not exposed to radiation had bacterial growth.
Radiation may be an effective method of sterilization for antibiotic impregnated beads.
目前推荐使用环氧乙烷对含抗生素的珠子进行灭菌；然而，这种方法对工作人员存在健康风险，且可用性越来越低。
对辐射灭菌含阿米卡星、恩诺沙星和头孢噻呋的聚甲基丙烯酸甲酯（PMMA）和巴黎石膏（POP）珠子的效果进行一项初步研究。
辐射能有效对珠子进行灭菌，且不影响抗生素的疗效。
以清洁但非无菌的方式制备PMMA和POP珠子，分别添加三种抗生素（阿米卡星、恩诺沙星、头孢噻呋）之一或不添加抗生素。然后将珠子分别接受总剂量为0千戈瑞（kGy）、10 kGy和25 kGy的辐射。将珠子在接种有大肠杆菌、金黄色葡萄球菌或铜绿假单胞菌的穆勒-欣顿琼脂平板上孵育24小时，或在脑心浸液肉汤中培养48小时。在琼脂平板上测量抑菌圈，并使用方差分析对抑菌圈直径进行统计分析。
所有PMMA珠子在不同辐射水平下的抑菌圈直径没有差异。POP珠子也是如此，但恩诺沙星除外，随着辐射水平增加，其抑菌圈显著减小，不过未评估这一发现的临床意义。只有未添加抗生素且未接受辐射的珠子有细菌生长。
辐射可能是对含抗生素珠子进行灭菌的有效方法。
