Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel.
Planta. 1979 Jan;145(3):273-8. doi: 10.1007/BF00454452.
Because of uniformity and small distances for transport, cell suspensions offer a system for rapid measurements of initial reactions of phytotoxic compounds. We had previously shown that a growth regulator, dikegulac (2,3:4,6 di-o-isopropylidine-2-keto-L-gulonate) inhibits amino acid incorporation into proteins. Using Solanum nigrum suspension cultures, it was found that dikegulac rapidly inhibits amino acid uptake into cells, before inhibiting incorporation, with time points starting at a few minutes, and kinetics that can be extrapolated back to time zero. With more rapid kinetics this compound induces leakage of a preloaded dye. The rate of leakage was less with stationary cells in suspension, reiterating that they are more resistant to the effects of this compound. It was thus concluded that at the concentrations used, the first effect of dikegulac (or one very close to the first effect) is on the cell membrane.
由于均匀性和运输距离短，细胞悬浮液提供了一种快速测量植物毒性化合物初始反应的系统。我们之前曾表明，一种生长调节剂，二氢古洛糖酸（2,3:4,6-二-O-异亚丙基-2-酮-L-古洛糖酸）抑制氨基酸掺入蛋白质。使用茄属悬浮培养物，发现二氢古洛糖酸在抑制掺入之前，迅速抑制氨基酸进入细胞，时间点从几分钟开始，动力学可以外推到零时间。这种化合物具有更快的动力学，会诱导预先加载染料的泄漏。在悬浮液中的静止细胞中泄漏的速度较慢，这再次表明它们对该化合物的作用更具抵抗力。因此得出的结论是，在使用的浓度下，二氢古洛糖酸（或非常接近第一个作用的化合物）的第一个作用是在细胞膜上。
