Institut für Forstbotanik der Universität Göttingen, Göttingen, Germany.
Planta. 1971 Dec;99(4):283-9. doi: 10.1007/BF00385820.
The patterns of multiple esterases from needles and macrogametophytes ofPicea abies were examined by polyacrylamide-gel disc-electrophoresis. There are three patterns: (1) two slow-migrating bands, (2) three fast-migrating bands, and (3) a combination of (1) and (2). Re-electrophoresis of the individual bands and genetic analysis demonstrated the existence of two distinct isozymes controlled by two alleles at one locus. In a native population the esterase patterns were in a Hardy-Weinberg equilibrium. In the offspring of crosses between clonal grafts which represent individual trees with different esterase patterns, the patterns segregated in Mendelian ratios. In the haploid macrogametophytes of individual trees they also segregated as expected, i.e. in a 1:1 ratio. This latter fact allows a genetic analysis of individual trees without crossing experiments by investigation of the isozyme patterns only.
采用聚丙烯酰胺凝胶圆盘电泳法研究了云杉针和大配子体的多种酯酶模式。有三种模式：（1）两条慢迁移带，（2）三条快迁移带，（3）模式（1）和（2）的组合。对单个条带的重新电泳和遗传分析表明，存在两种由一个位点上的两个等位基因控制的不同同工酶。在一个自然种群中，酯酶模式处于哈迪-温伯格平衡状态。在由具有不同酯酶模式的单个树木的克隆嫁接代表的个体树木的杂交后代中，模式按孟德尔比例分离。在个别树木的单倍体大配子体中，它们也按预期分离，即 1:1 比例。这一事实使得可以仅通过同工酶模式的研究而无需杂交实验即可对个别树木进行遗传分析。
