Centro di Studio del C.N.R. per la Biologia Cellulare e Molecolare delle Piante, Istituto di Scienze Botaniche dell'Università, via Giuseppe Colombo 60, I-20133, Milano, Italy.
Planta. 1979 Jan;146(1):19-23. doi: 10.1007/BF00381250.
During germination, the transmembrane electric potential (PD) of cortical cells of the embryonal axis of radish seeds (Raphanus sativus L.) rises from-120 mV initially to a maximum of-150 mV after 5 h incubation, then falls again to stable values of around-120 mV. Treatments inhibiting germination block the transitory PD increase. Administration of uncoupling agents or low temperatures, during the process of germination, produces a marked fall of the PD transitory increase. Abscisic Acid has a parallel inhibitory effect on PD and germination, while fusicoccin produces a rise in both; administration of abscisic acid with fusicoccin inhibits germination, while the PD remains at the high levels given by fusicoccin. These results are discussed in relation to ion exchange at membrane level.
在发芽过程中,萝卜(Raphanus sativus L.)胚轴皮质细胞的跨膜电势(PD)从最初的-120 mV 上升到 5 小时孵育后的最大值-150 mV,然后再次下降到稳定值约-120 mV。抑制发芽的处理阻止了瞬时 PD 增加。在发芽过程中施用解偶联剂或低温会导致 PD 瞬时增加明显下降。脱落酸(ABA)对 PD 和发芽具有平行的抑制作用,而赤霉素(fusicoccin)则会同时引起两者的增加;用脱落酸和赤霉素处理会抑制发芽,而 PD 则保持赤霉素产生的高水平。这些结果与膜水平的离子交换有关。