Evins W H, Varner J E
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1631-3. doi: 10.1073/pnas.68.7.1631.
The rate of synthesis of the endoplasmic reticulum in barley aleurone cells after treatment with gibberellic acid was determined by measurement of [(14)C]-choline incorporation into acid-insoluble material in a semipurified fraction containing the endoplasmic reticulum. 94% of the (14)C incorporated into this fraction is extractable by lipid solvents and only 9% is removed by procedures for nucleic acid extraction. Gibberellic acid increases the rate of synthesis of the endoplasmic reticulum 4- to 8-fold, starting about 4 hr after addition of the hormone (at about the same time as polysome formation). Abscisic acid inhibits this gibberellic acid-enhanced increase in the rate of synthesis of endoplasmic reticulum.
通过测量[（14）C]-胆碱掺入含有内质网的半纯化组分中的酸不溶性物质，来测定用赤霉素处理后大麦糊粉层细胞内质网的合成速率。掺入该组分中的（14）C有94%可被脂质溶剂提取，只有9%可通过核酸提取程序去除。赤霉素可使内质网的合成速率提高4至8倍，在添加激素后约4小时开始（与多核糖体形成的时间大致相同）。脱落酸抑制赤霉素增强的内质网合成速率的增加。
