John Innes Institute, Colney Lane, NR4 7UH, Norwich, U.K..
Planta. 1979 Jan;146(2):203-10. doi: 10.1007/BF00388233.
A study has been made of the wall fibrils produced by tobacco protoplasts, using scanning electron microscopy in conjunction with negative staining. It has been shown that the fibres seen in scanning electron microscopy correspond to aggregates of microfibrils. These aggregates are only visible where they are lifted clear of the protoplast surface. Negative staining of fixed protoplasts shows that the aggregation of microfibrils into the fibres visible in scanning electron microscopy is probably produced by air-drying. Gentle disruption of microfibrils produces both random broken fragments and bundles of short pieces of fibrillar material about 60 nm in length. This material is present in undisrupted young walls, but not in undisrupted older walls. The microfibrils in young walls seem much more fragile and liable to breakage than those in older walls. These results are discussed in terms of the interpretation of scanning electron microscope images and the mechanism of cellulose microfibril formation by higher plants.
使用扫描电子显微镜结合负染色技术研究了烟草原生质体产生的壁原纤维。结果表明，在扫描电子显微镜中观察到的纤维与微原纤维的聚集物相对应。这些聚集物仅在它们从原生质体表面抬起时可见。固定原生质体的负染色显示，微原纤维聚集到扫描电子显微镜中可见的纤维中，可能是由风干引起的。轻轻破坏微原纤维会产生随机的断裂碎片和大约 60nm 长的短纤维状物质束。这种物质存在于未被破坏的年轻壁中，但不存在于未被破坏的较老壁中。年轻壁中的微原纤维似乎比老壁中的更脆弱，更容易断裂。这些结果是根据扫描电子显微镜图像的解释和高等植物纤维素微原纤维形成的机制进行讨论的。
