McMillan T J, Hart I R
Clin Exp Metastasis. 1986 Oct-Dec;4(4):285-92. doi: 10.1007/BF00133593.
The effect of in vitro pretreatment of B16 murine melanoma cells with various cancer chemotherapeutic agents on their subsequent experimental metastatic capacity has been examined. Methotrexate, cytosine arabinoside, 5-azacytidine and aphidicolin all produced significant increases in the number of lung nodules formed following the i.v. injection of tumour cells. This effect was not seen with melphalan or 5-fluorouracil. Since the doses of melphalan and 5-fluorouracil used produced similar levels of cell kill to cytosine arabinoside and aphidicolin it appears that a cytotoxic effect was not sufficient to produce increased lung nodule formation. Analysis of the perturbations in the cell cycle induced by the drugs did not reveal any consistent differences between those drugs which enhanced experimental metastasis and those which did not. The precise mechanisms underlying this phenomenon remain to be elucidated but the results are consistent with the possibility that anticancer agents may play a role in assisting tumour progression.
研究了用各种癌症化疗药物对B16小鼠黑色素瘤细胞进行体外预处理对其随后实验性转移能力的影响。甲氨蝶呤、阿糖胞苷、5-氮杂胞苷和阿非迪霉素在静脉注射肿瘤细胞后，均使形成的肺结节数量显著增加。美法仑或5-氟尿嘧啶则未观察到这种效果。由于所用美法仑和5-氟尿嘧啶的剂量产生的细胞杀伤水平与阿糖胞苷和阿非迪霉素相似，因此看来细胞毒性作用不足以导致肺结节形成增加。对药物诱导的细胞周期扰动的分析未发现增强实验性转移的药物与未增强实验性转移的药物之间存在任何一致的差异。这一现象背后的确切机制仍有待阐明，但结果与抗癌药物可能在协助肿瘤进展中起作用的可能性是一致的。
