Institute of Biochemistry, Odense University, Campusvej 55, DK-5230, Odense M, Denmark.
Photosynth Res. 1993 Feb;35(2):171-8. doi: 10.1007/BF00014747.
Thylakoid membranes from spinach were fragmented mechanically and separated into vesicles originating from grana and stroma-exposed lamellae (Andreasson et al. (1988) Biochim Biophys Acta 936: 339-350). The grana vesicles were further fragmented and separated into smaller vesicles originating from different parts of the grana (Svensson and Albertsson (1989) Photosynth Res 20: 249-259). All vesicles so obtained were analyzed with respect to chlorophyll and carotenoid composition by reverse phase HPLC. For all fractions the following relations (mole/mole) were found: 1 carotenoid per 4 chlorophyll (a+b), 2 lutein per 5 chlorophyll b and 5 violaxanthin per 100 chlorophyll (a + b). The contents of lutein and neoxanthin were each linearly related to chlorophyll b and β-carotene was linearly related to chlorophyll a.
菠菜类囊体膜经机械破碎后，分离成来源于基质暴露类囊体和基粒的小泡（Andreasson 等人，1988 年，《生物化学与生物物理学报》936:339-350）。基粒小泡进一步破碎，分离成来源于不同基粒部分的更小的小泡（Svensson 和 Albertsson，1989 年，《光合作用研究》20:249-259）。所有获得的小泡均通过反相 HPLC 分析叶绿素和类胡萝卜素组成。对于所有的小泡，发现以下比例关系（摩尔/摩尔）：1 个类胡萝卜素对应 4 个叶绿素（a+b），2 个叶黄素对应 5 个叶绿素 b，5 个紫黄质对应 100 个叶绿素（a+b）。叶黄素和新黄质的含量与叶绿素 b 均呈线性相关，β-胡萝卜素与叶绿素 a 呈线性相关。
