Mazlan Nor Aishah, Mediani Ahmed, Abas Faridah, Ahmad Syahida, Shaari Khozirah, Khamis Shamsul, Lajis N H
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
ScientificWorldJournal. 2013 Nov 4;2013:312741. doi: 10.1155/2013/312741. eCollection 2013.
The methanol extracts of three Macaranga species (M. denticulata, M. pruinosa, and M. gigantea) were screened to evaluate their total phenolic contents and activities as cholinesterase inhibitors, nitric oxide (NO) production inhibitors, tyrosinase inhibitors, and antioxidants. The bark of M. denticulata showed the highest total phenolic content (2682 mg gallic acid equivalent (GAE)/100 g) and free radical scavenging activity (IC50 = 0.063 mg/mL). All of the samples inhibited linoleic acid peroxidation by greater than 80%, with the leaves of M. gigantea exhibiting the highest inhibition of 92.21%. Most of the samples exhibited significant antioxidant potential. The bark of M. denticulata and the leaves of both M. pruinosa and M. gigantea exhibited greater than 50% tyrosinase inhibition, with the bark of M. denticulata having the highest percentage of inhibition (68.7%). The bark and leaves of M. denticulata exhibited greater than 50% inhibition (73.82% and 54.50%, resp.) of the acetylcholinesterase enzyme (AChE), while none of the samples showed any significant inhibition of butyrylcholinesterase (BChE). Only the bark of M. denticulata and M. gigantea displayed greater than 50% inhibition of nitric oxide production in cells (81.79% and 56.51%, resp.). These bioactivities indicate that some Macaranga spp. have therapeutic potential in medicinal research.
对三种血桐属植物（齿叶血桐、粉叶血桐和大血桐）的甲醇提取物进行了筛选，以评估其总酚含量以及作为胆碱酯酶抑制剂、一氧化氮（NO）生成抑制剂、酪氨酸酶抑制剂和抗氧化剂的活性。齿叶血桐的树皮显示出最高的总酚含量（2682毫克没食子酸当量（GAE）/100克）和自由基清除活性（IC50 = 0.063毫克/毫升）。所有样品对亚油酸过氧化的抑制率均大于80%，其中大血桐的叶子抑制率最高，为92.21%。大多数样品表现出显著的抗氧化潜力。齿叶血桐的树皮以及粉叶血桐和大血桐的叶子对酪氨酸酶的抑制率均大于50%，其中齿叶血桐树皮的抑制率最高（68.7%）。齿叶血桐的树皮和叶子对乙酰胆碱酯酶（AChE）的抑制率均大于50%（分别为73.82%和54.50%），而所有样品对丁酰胆碱酯酶（BChE）均未表现出显著抑制作用。只有齿叶血桐和大血桐的树皮对细胞中一氧化氮生成的抑制率大于50%（分别为81.79%和56.51%）。这些生物活性表明，一些血桐属植物在医学研究中具有治疗潜力。
