Department of Nutrition and Food Hygiene, School of Public Health, Peking University, No. 38 Xueyuan Road, Haidian District, Beijing 100191, China.
Biomed Res Int. 2013;2013:204187. doi: 10.1155/2013/204187. Epub 2013 Nov 11.
The objective of this study was to assess whether nucleotides supplementation in vitro could suppress ethanol-induced developmental toxicity in mouse. The models of whole embryo culture (WEC) and midbrain (MB) cell micromass culture were used in this study. In WEC system, exposure to 4.0 mg/mL ethanol for 48 h yielded various developmental malformations of the mice embryos. Nucleotides supplementation (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) improved the growth parameters to some extent, and the protective effects peaked at 4.00 mg/L. In MB cell micromass culture system, exposure to 4.0 mg/mL ethanol for 5 days resulted in suppression of proliferation and differentiation. Supplementation of nucleotides (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) showed some protective effects, which peaked at 4.00 mg/L, too. The present research indicated that nucleotides supplementation might be of some benefit in the prevention of ethanol-induced birth defects; however, appropriate dosage requires attention.
本研究旨在评估核苷酸体外补充是否能抑制乙醇诱导的小鼠胚胎发育毒性。本研究采用全胚胎培养（WEC）和中脑神经细胞微团培养模型。在 WEC 系统中，暴露于 4.0mg/mL 乙醇 48 小时会导致小鼠胚胎出现各种发育畸形。核苷酸（0.16、0.80、4.00、20.00 和 100.00mg/L）补充在一定程度上改善了生长参数，保护作用在 4.00mg/L 时达到峰值。在 MB 细胞微团培养系统中，暴露于 4.0mg/mL 乙醇 5 天会抑制增殖和分化。核苷酸（0.16、0.80、4.00、20.00 和 100.00mg/L）补充也表现出一定的保护作用，在 4.00mg/L 时达到峰值。本研究表明，核苷酸补充可能有助于预防乙醇引起的出生缺陷，但需要注意适当的剂量。
