Xu Yajun, Tang Yunan, Li Yong
Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100083, China.
Br J Nutr. 2008 Mar;99(3):455-61. doi: 10.1017/S0007114507812074. Epub 2007 Aug 13.
Maternal alcohol consumption during pregnancy can induce central nervous system abnormalities in the fetus, and folic acid supplementation can reverse some of the effects. The objective of the present study was to investigate prenatal alcohol exposure-induced fetal brain proteome alteration and the protective effect of folic acid using proteomic techniques. Alcohol (5.0 g/kg) was given intragastrically from gestational day (GD) 6 to 15, with or without 60.0 mg folic acid/kg given intragastrically during GD 1-16 to pregnant Balb/c mice. The control group received distilled water only. Results of litter evaluation on GD 18 showed that supplementation of folic acid reversed the prevalence of microcephaly induced by alcohol. Proteomic analysis indicated that, under the dosage of the present investigation, folic acid mainly reversed the alcohol-altered proteins involved in energy production, signal pathways and protein translation, which are all important for central nervous system development.
孕期母亲饮酒可导致胎儿中枢神经系统异常，而补充叶酸可逆转部分影响。本研究的目的是利用蛋白质组学技术研究产前酒精暴露引起的胎儿脑蛋白质组改变以及叶酸的保护作用。在妊娠第6天至15天，给怀孕的Balb/c小鼠灌胃给予酒精（5.0 g/kg），在妊娠第1天至16天期间，同时或不同时灌胃给予60.0 mg叶酸/kg。对照组仅给予蒸馏水。妊娠第18天的窝仔评估结果表明，补充叶酸可逆转酒精诱导的小头畸形发生率。蛋白质组分析表明，在本研究剂量下，叶酸主要逆转了参与能量产生、信号通路和蛋白质翻译的酒精改变蛋白，这些对中枢神经系统发育都很重要。
