Petrova E S, Isaeva E N, Korzhevskii D E
Institute of Experimental Medicine, North-Western Branch of the Russian Academy of Medical Sciences; State Research Institute of Highly Pure Biopreparations, Federal Medical and Biological Agency of Russia, St. Petersburg, Russia.
Bull Exp Biol Med. 2013 Nov;156(1):136-8. doi: 10.1007/s10517-013-2296-9.
Fragments of the dorsolateral wall of the anterior brain vesicle from rat embryos on embryonic day 15 were dissociated and the resultant suspension containing single cells and cell aggregates was injected into the proximal segment of crushed sciatic nerve of adult animals for evaluation of their engrafting and differentiation under conditions of changed microenvironment. On days 1 and 21 postoperation, Msi-1, GFAP, NeuN, vimentin, and PCNA were detected by immunohistochemical methods. Small clusters of Msi-1-immunopositive cells were detected in the nerve trunks on the next day after transplantation. On day 21 after surgery, these precursors differentiate into nerve cells, astrocytes, and primarily ependymocytes.
取胚胎第15天大鼠胚胎前脑泡背外侧壁的碎片进行解离，将所得含有单细胞和细胞聚集体的悬液注射到成年动物坐骨神经挤压伤的近端，以评估其在微环境改变条件下的植入和分化情况。术后第1天和第21天，采用免疫组织化学方法检测Msi-1、GFAP、NeuN、波形蛋白和PCNA。移植后第2天在神经干中检测到小簇Msi-1免疫阳性细胞。术后第21天，这些前体细胞分化为神经细胞、星形胶质细胞，主要是室管膜细胞。
