Ivanyi J, Sharp K
Immunology. 1986 Nov;59(3):329-32.
The genetic control of antibody responses after immunization with Mycobacterium tuberculosis soluble antigens was examined in inbred and H-2 congenic mouse strains. Antibody levels to five distinct epitopes were determined by a competitive inhibition test using radiolabelled murine monoclonal probes. High or low responder antibody levels were associated with either the H-2b (TB23, TB71 and TB72 specificities) or the H-2k (TB68) allele on both B10 and BALB backgrounds. The high response of TB78 specificity associated with the H-2k on the BALB but with H-2b haplotype on B10 background. The phenotype of (C57BL/6 X CBA)F1 hybrids reflected the high response for four and the low or intermediary response for one (TB68) of the tested paratopes. This is the first demonstration of immune response gene control in respect of defined mycobacterial protein epitopes. The implications towards the analysis of pathogenic or protective mechanisms during mycobacterial infection are briefly outlined.
在用结核分枝杆菌可溶性抗原免疫后，对近交系和H - 2同源小鼠品系中抗体应答的遗传控制进行了研究。使用放射性标记的鼠单克隆探针，通过竞争抑制试验测定了针对五个不同表位的抗体水平。在B10和BALB背景下，高或低应答抗体水平分别与H - 2b（TB23、TB71和TB72特异性）或H - 2k（TB68）等位基因相关。TB78特异性的高应答在BALB背景下与H - 2k相关，但在B10背景下与H - 2b单倍型相关。（C57BL/6×CBA）F1杂种的表型反映了所测试的四个互补决定区的高应答以及一个（TB68）的低或中等应答。这是首次证明针对特定分枝杆菌蛋白表位的免疫应答基因控制。简要概述了其对分枝杆菌感染期间致病或保护机制分析的意义。
