Peng Rui, Li Dan-Di, Cai Kun, Qin Jia-Jian, Wang Yong-Xia, Lin Qiang, Guo Yan-Qing, Zhao Chun-Yan, Duan Zhao-Jun
Jilin University, Changchun, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013 Jun;27(3):164-6.
To study the epdimiology characteristics and the diversity of VP6 gene of GCRV in Lulong, and to provide the basis for GCRV in-depth research.
793 stool specimens from porcine with diarrhea or not from Lulong in 2007 and 2008. GCRV was detected by nested multiple reverse transcription- polymerase chain reaction (nested RT-PCR) , and analyzed the identity and conducted phylogenetic tree by the seqences.
The positive rate of GCRV was 16.65%. Porcine GCRV strains of Lulong had significant homology differences. Phylogenetic analysis indicated porcine GCRVs were with significant diversity. Amino acid analysis showed GCRV strains with the same host shared the nearest kinship.
The infection rate of GCRV was high from 2007 to 2008 in Lulong. Homology and phylogenetic analysis showed that VP6 gene diversity was widespread. The experimental data provided basis for molecular characteristics of porcine GCRVs.
研究卢龙地区草鱼呼肠孤病毒（GCRV）的流行病学特征及VP6基因的多样性，为GCRV的深入研究提供依据。
采集2007年和2008年卢龙地区793份腹泻或未腹泻猪的粪便样本。采用巢式多重逆转录-聚合酶链反应（nested RT-PCR）检测GCRV，并对其序列进行同源性分析和构建系统发育树。
GCRV阳性率为16.65%。卢龙地区猪GCRV毒株存在显著的同源性差异。系统发育分析表明猪GCRV具有显著的多样性。氨基酸分析显示相同宿主的GCRV毒株亲缘关系最近。
2007—2008年卢龙地区GCRV感染率较高。同源性和系统发育分析表明VP6基因多样性广泛。本实验数据为猪GCRV的分子特征研究提供了依据。
