Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.
J Gen Virol. 2011 Feb;92(Pt 2):361-9. doi: 10.1099/vir.0.027375-0. Epub 2010 Nov 3.
Group C rotavirus (GCRV) is distributed worldwide as an enteric pathogen in humans and animals. However, to date, whole-genome sequences are available only for a human strain (Bristol) and a porcine strain (Cowden). To investigate the genetic diversity of human GCRVs, nearly full-length sequences of all 11 RNA segments were determined for human GCRVs detected recently in India (v508), Bangladesh (BS347), China (Wu82 and YNR001) and Japan (OH567 and BK0830) and analysed phylogenetically with sequence data for GCRVs published previously. All the RNA segments of human GCRV strains except for the VP3 gene showed high levels of conservation (>93 % nucleotide sequence identity, >92 % amino acid sequence identity), belonging to a single genetic cluster distinct from those of animal GCRVs. In contrast, the VP3 genes of human GCRVs could be discriminated into two clusters, designated M2 and M3, that were distinguished phylogenetically from those of porcine and bovine GCRVs (clusters M1 and M4, respectively). Between M2 and M3, amino acid sequence identity of the VP3 gene was 84.1-84.7 %, whereas high identities were observed within each cluster (92.3-97.6 % for M2, 98.2-99.3 % for M3). Sequence divergence among the four VP3 clusters was observed throughout the amino acid sequence except for conserved motifs, including those possibly related to enzyme functions of VP3. The presence of obvious genetic diversity only in the VP3 gene among human GCRVs suggested that either the M2 or M3 VP3 gene of human GCRVs might have been derived through reassortment from an animal GCRV or from an unidentified human GCRV strain belonging to a novel genogroup.
C 组轮状病毒（GCRV）作为一种肠病原体分布于全球各地的人类和动物中。然而，迄今为止，全基因组序列仅可用于人类菌株（布里斯托尔）和猪菌株（考登）。为了研究人类 GCRV 的遗传多样性，我们对最近在印度（v508）、孟加拉国（BS347）、中国（Wu82 和 YNR001）和日本（OH567 和 BK0830）检测到的人类 GCRV 进行了所有 11 个 RNA 片段的近乎全长序列测定，并与先前发表的 GCRV 序列数据进行了系统进化分析。除 VP3 基因外，所有人类 GCRV 株的 RNA 片段均表现出高度的保守性（>93%核苷酸序列同一性，>92%氨基酸序列同一性），属于与动物 GCRV 不同的单一遗传簇。相比之下，人类 GCRV 的 VP3 基因可分为两个簇，命名为 M2 和 M3，这两个簇在系统进化上与猪和牛 GCRV 的 VP3 基因簇（分别为 M1 和 M4）不同。在 M2 和 M3 之间，VP3 基因的氨基酸序列同一性为 84.1-84.7%，而在每个簇内观察到高度同一性（M2 为 92.3-97.6%，M3 为 98.2-99.3%）。在四个 VP3 簇之间观察到整个氨基酸序列的序列差异，除了保守基序外，包括那些可能与 VP3 的酶功能相关的基序。在人类 GCRV 中仅在 VP3 基因中存在明显的遗传多样性表明，人类 GCRV 的 M2 或 M3 VP3 基因可能是通过来自动物 GCRV 的重组或来自未知的属于新型基因群的人类 GCRV 株而产生的。
