March Rosselló Gabriel A, Gutiérrez Rodríguez María P, de Lejarazu Leonardo Raúl Ortiz, Orduña Domingo Antonio, Bratos Pérez Miguel A
Service of Microbiology, University Clinic Hospital of Valladolid, Valladolid, Spain.
APMIS. 2014 Sep;122(9):790-5. doi: 10.1111/apm.12208. Epub 2013 Dec 10.
Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry is a widely used proteomic technique in clinical microbiology laboratories, and enables microbial identification directly from clinical samples. This study seeks to establish a protocol for bacterial identification from monomicrobial urine samples that have tested positive in the screening with Sysmex UF-1000i (Sysmex Corporation, Kobe, Japan). Sysmex UF-1000i counts ≥1 × 10(7) bacteria/mL indicate a sufficient bacterial concentration to allow direct identification from urine, with 87.5% sensitivity. Microbial identification from urine with Sysmex UF-1000i counts between 1 × 10(5) and 1 × 10(7) bacteria/ml requires preincubation to obtain the adequate amount of bacteria needed for analysis, and 91.7% sensitivity thus being achieved.
基质辅助激光解吸/电离飞行时间质谱是临床微生物实验室中广泛使用的蛋白质组学技术，可直接从临床样本中鉴定微生物。本研究旨在建立一种从经Sysmex UF-1000i（Sysmex公司，日本神户）筛查呈阳性的单一微生物尿液样本中鉴定细菌的方案。Sysmex UF-1000i计数≥1×10⁷细菌/mL表明细菌浓度足以直接从尿液中进行鉴定，灵敏度为87.5%。对于Sysmex UF-1000i计数在1×10⁵至1×10⁷细菌/mL之间的尿液样本进行微生物鉴定，需要进行预培养以获得分析所需的足够数量的细菌，从而实现91.7%的灵敏度。
