Taffouo Victor Désiré, Ngwene Benard, Akoa Amougou, Franken Philipp
Department of Botany, Faculty of Science, University of Douala, P.O. Box 24157, Douala, Cameroon,
Mycorrhiza. 2014 Jul;24(5):361-8. doi: 10.1007/s00572-013-0544-5. Epub 2013 Dec 10.
The present study was undertaken to evaluate the effects of phosphorus (P) application and arbuscular mycorrhizal (AM) fungi (Funneliformis mosseae) on growth, foliar nitrogen mobilization, and phosphorus partitioning in cowpea (Vigna unguiculata cv. Vita-5) plants. The experiment was conducted in a greenhouse in pots containing a mixture of vermiculite and sterilized quartz sand. Mycorrhizal and non-mycorrhizal cowpea plants were supplied with three levels of soluble P (0.1 (low P), 0.5 (medium P), or 1.0 mM (high P)).Cowpea plants supplied with low P fertilization showed significantly (p < 0.05) higher root colonization than those with medium and high P fertilization at both the vegetative and pod-filling stages. P uptake and growth parameters of cowpea plants were positively influenced by mycorrhizal inoculation only in the medium P fertilization treatment at the vegetative stage. Lack of these effects in the other treatments may be linked to either a very low P supply (in the low P treatment at the vegetative stage) or the availability of optimal levels of freely diffusible P in the substrate towards the pod-filling stage due to accumulation with time. The N concentration in leaves of all cowpea plants were lower at the pod-filling stage than at the vegetative stage, presumably as a result of N mobilization from vegetative organs to the developing pods. This was however not influenced by AM fungal inoculation and may be a consequence of the lack of an improved plant P acquisition by the fungus at the pod-filling stage.
本研究旨在评估施磷及接种丛枝菌根（AM）真菌（摩西管柄囊霉）对豇豆（品种Vita - 5）植株生长、叶片氮素转运及磷素分配的影响。试验在温室中装有蛭石和灭菌石英砂混合物的花盆中进行。给接种菌根和未接种菌根的豇豆植株分别供应三种水平的可溶性磷（0.1 mM（低磷）、0.5 mM（中磷）或1.0 mM（高磷））。在营养生长阶段和结荚期，低磷施肥的豇豆植株的根系定殖率显著高于（p < 0.05）中磷和高磷施肥的植株。仅在营养生长阶段的中磷施肥处理中，接种菌根对豇豆植株的磷吸收和生长参数有积极影响。其他处理中未出现这些影响可能与极低的磷供应（营养生长阶段的低磷处理）或由于随着时间积累，在结荚期基质中可自由扩散的磷达到最佳水平有关。所有豇豆植株叶片中的氮浓度在结荚期均低于营养生长阶段，这可能是由于氮从营养器官转运至发育中的豆荚。然而，这不受AM真菌接种的影响，可能是因为在结荚期真菌未能改善植株对磷的吸收。
