Prof. Dr., Small Animal Clinic, University of Veterinary Medicine Hannover, Bünteweg 9, 30550 Hannover, Germany.
Anticancer Res. 2013 Dec;33(12):5421-31.
BACKGROUND/AIM: In human prostate cancer cells with a stem cell-like character (cancer stem cells, CSC) are considered to play a major role in disease development, progression and relapse. Aim of the study was to evaluate if similar cells are present and active in canine prostate cancer providing a naturally-occurring mammalian model for the development of therapeutic approaches targeting CSC.
Stem cell marker expression of CD133, CD44, C-KIT, CD34, ITGA6, OCT4, DDX5 and MELK in canine prostate carcinomas and prostate cyst cell lines were screened by Polymerase Chain Reaction (PCR), quantitative Polymerase Chain Reaction (qPCR) and partially analysed by flow cytometry.
Marker analyses by PCR and qPCR, revealed a complex expression pattern for the analysed marker genes, providing a characteristic marker pattern for the studied cell lines. Thereby CD44, CD133, ITGA6 and DDX5 showed the most prominent expression in the analysed cell lines.
The results revealed a characteristic stem cell marker expression in the analysed cell lines, indicating the presence of CSC in canine prostate cancer.
背景/目的：在具有干细胞样特征的人类前列腺癌细胞（癌症干细胞，CSC）中，它们被认为在疾病的发展、进展和复发中起着重要作用。本研究的目的是评估在犬前列腺癌中是否存在类似的活跃的干细胞样细胞，为开发针对 CSC 的治疗方法提供一种自然发生的哺乳动物模型。
通过聚合酶链反应（PCR）、实时聚合酶链反应（qPCR）筛选犬前列腺癌和前列腺囊泡细胞系中 CD133、CD44、C-KIT、CD34、ITGA6、OCT4、DDX5 和 MELK 等干细胞标志物的表达，并部分通过流式细胞术进行分析。
通过 PCR 和 qPCR 分析，发现分析标记基因的表达模式复杂，为研究的细胞系提供了特征性的标记模式。其中 CD44、CD133、ITGA6 和 DDX5 在分析的细胞系中表达最为显著。
研究结果显示，在分析的细胞系中存在特征性的干细胞标志物表达，提示犬前列腺癌中存在 CSC。
