Noureen Farah, Khan Muhammad Rashid, Shah Naseer Ali, Khan Rahmat Ali, Naz Kiran, Sattar Saadia
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Asian Pac J Trop Med. 2017 Apr;10(4):380-389. doi: 10.1016/j.apjtm.2017.03.027. Epub 2017 May 2.
To evaluate in vitro and in vivo antioxidant potency of Pistacia chinensis (P. chinensis) bark and leaves extracts along with its protective role against CCl induced toxicity in testis of the rat.
Various in vitro models such as DPPH, ABTS, hydrogen peroxide, superoxide, hydroxyl and nitric oxide scavenging activities, anti-lipid peroxidation activity, phospho-molybdenum activity, β carotene bleaching assay was used for analysis of antioxidant potential. Experimental groups for in vivo study were: Group Ⅰ (control) untreated, Group Ⅱ (Vehicle control), Group Ⅲ (1 mL/kg b.w 30% CCl), Group Ⅳ (1 mL/kg b.w CCl + Silymarin), Group Ⅴ (200 mg/kg b.w PCBE + CCl), Group Ⅵ (400 mg/kg b.w PCBE + CCl) and Group Ⅶ (400 mg/kg b.w PCBE alone).
In vitro antioxidant assays displayed significant results and the highest activity was not specified to a specific extract. However, ethyl acetate extract of bark (PCBE) showed highest results in most of the antioxidant assays i.e. beta-carotene bleaching, hydroxyl radical scavenging, ABTS, lipid peroxidation and superoxide radical scavenging activity. On this base, this fraction was selected for in vivo antioxidant experiment. Testis tissues were analyzed to observe the protective effects of PCBE on antioxidant enzymes; catalase, superoxide dismutase, peroxidase, glutathione-S-transferase, glutathione reductase, glutathione peroxidase and quinone reductase activities and glutathione (GSH) as well as nitrite content. Profile of plasma testosterone was also compared to various treatments. Observation suggests a protective role of P. chinensis against CCl induced toxicity.
It is concluded that some bioactive antioxidants of P. chinensis bark might be a good source to isolate the potent antioxidant components.
评估黄连木树皮和树叶提取物的体外和体内抗氧化能力，以及其对四氯化碳诱导的大鼠睾丸毒性的保护作用。
采用多种体外模型，如DPPH、ABTS、过氧化氢、超氧化物、羟基和一氧化氮清除活性、抗脂质过氧化活性、磷钼活性、β-胡萝卜素漂白试验，分析抗氧化潜力。体内研究的实验组为：Ⅰ组（对照组）未处理，Ⅱ组（溶剂对照组），Ⅲ组（1 mL/kg体重30%四氯化碳），Ⅳ组（1 mL/kg体重四氯化碳+水飞蓟宾），Ⅴ组（200 mg/kg体重黄连木树皮乙酸乙酯提取物+PCCl），Ⅵ组（400 mg/kg体重黄连木树皮乙酸乙酯提取物+PCCl）和Ⅶ组（400 mg/kg体重黄连木树皮乙酸乙酯提取物单独使用）。
体外抗氧化试验显示出显著结果，且最高活性并非特定于某一种提取物。然而，树皮乙酸乙酯提取物（PCBE）在大多数抗氧化试验中表现出最高结果，即β-胡萝卜素漂白、羟基自由基清除、ABTS、脂质过氧化和超氧阴离子自由基清除活性。基于此，该组分被选用于体内抗氧化实验。分析睾丸组织以观察PCBE对抗氧化酶的保护作用；过氧化氢酶、超氧化物歧化酶、过氧化物酶、谷胱甘肽-S-转移酶、谷胱甘肽还原酶、谷胱甘肽过氧化物酶和醌还原酶活性以及谷胱甘肽（GSH）和亚硝酸盐含量。还比较了不同处理下血浆睾酮水平。观察结果表明黄连木对四氯化碳诱导的毒性具有保护作用。
得出结论，黄连木树皮中的一些生物活性抗氧化剂可能是分离有效抗氧化成分的良好来源。
