Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare (ICRM), CNR, Via Mario Bianco, 9, 20131, Milano, Italy.
Analyst. 2014 Feb 7;139(3):528-42. doi: 10.1039/c3an01619f.
The nature of protein microarray platforms is favorable for multiplexing, leading to the development of tools for personalised medicine and highly precise diagnostics. However, to date, only a limited number of protein microarrays are available in the in vitro diagnostics (IVD) market. This review article will focus on the following operational challenges that are crucial for the use of microarrays in clinical settings: (1) probe printing and quality control; (2) procurement of bio-reagents and antibody cross-reactivity; (3) mass transport limitations and assay automation; (4) calibration and quantification. A selection of microarray assays applicable to IVD and a summary of the diagnostic products currently available on the market are provided.
蛋白质微阵列平台的性质有利于多重分析，从而开发出用于个性化医疗和高度精确诊断的工具。然而，迄今为止，体外诊断（IVD）市场上可用的蛋白质微阵列数量有限。本文将重点介绍在临床环境中使用微阵列的以下操作挑战：（1）探针打印和质量控制；（2）生物试剂的采购和抗体交叉反应性；（3）质量传输限制和分析自动化；（4）校准和定量。本文提供了适用于 IVD 的微阵列分析选择以及当前市场上可用诊断产品的摘要。
