Swidsinski Alexander, Loening-Baucke Vera, Mendling Werner, Dörffel Yvonne, Schilling Johannes, Halwani Zaher, Jiang Xue-feng, Verstraelen Hans, Swidsinski Sonja
Charité Hospital, CCM, Laboratory for Molecular Genetics, Polymicrobial Infections and Bacterial Biofilms and Department of Medicine, Gastroenterology, Universitätsmedizin Berlin, Germany.
Charité Hospital, CCM, Laboratory for Molecular Genetics, Polymicrobial Infections and Bacterial Biofilms and Department of Medicine, Gastroenterology, Universitätsmedizin Berlin.
Histol Histopathol. 2014 May;29(5):567-87. doi: 10.14670/HH-29.10.567. Epub 2013 Dec 11.
We analysed data on bacterial vaginosis (BV) contradicting the paradigm of mono-infection.
Tissues and epithelial cells of vagina, uterus, fallopian tubes and perianal region were investigated using fluorescence in situ hybridization (FISH) in women with BV and controls.
Healthy vagina was free of biofilms. Prolific structured polymicrobial (StPM) Gardnerella-dominated biofilm characterised BV. The intact StPM-Gardnerella-biofilm enveloped desquamated vaginal/prepuce epithelial cells and was secreted with urine and sperma. The disease involved both genders and occurred in pairs. Children born to women with BV were negative. Monotherapy with metronidazole, moxifloxacin or local antiseptics suppressed but often did not eradicate StPM-Gardnerella-biofilms. There was no BV without Gardnerella, but Gardnerella was not BV. Outside of StPM-biofilm, Gardnerella was also found in a subset of children and healthy adults, but was dispersed, temporal and did not transform into StPM-Gardnerella-biofilm.
StPM-Gardnerella-biofilm is an infectious subject. The assembly of single players to StPM-Gardnerella-biofilm is a not trivial every day process, but probably an evolutionary event with a long history of growth, propagation and selection for viability and ability to reshape the environment. The evolutionary memory is cemented in the structural differentiation of StPM-Gardnerella-biofilms and imparts them to resist previous and emerging challenges.
我们分析了与单一感染范式相矛盾的细菌性阴道病（BV）数据。
对患有BV的女性及其对照者，使用荧光原位杂交（FISH）技术研究阴道、子宫、输卵管和肛周区域的组织及上皮细胞。
健康阴道无生物膜。以加德纳菌为主的多微生物结构化生物膜（StPM）是BV的特征。完整的StPM - 加德纳菌生物膜包裹着脱落的阴道/包皮上皮细胞，并随尿液和精液排出。该疾病涉及两性且成对出现。BV女性所生子女检测为阴性。甲硝唑、莫西沙星或局部防腐剂的单一疗法可抑制但通常无法根除StPM - 加德纳菌生物膜。没有加德纳菌就没有BV，但有加德纳菌并不意味着就是BV。在StPM生物膜之外，加德纳菌也在一部分儿童和健康成年人中被发现，但呈分散状态，且短暂存在，不会转化为StPM - 加德纳菌生物膜。
StPM - 加德纳菌生物膜是一种感染源。单个成分组装成StPM - 加德纳菌生物膜并非日常简单过程，而可能是一个经历了漫长生长、繁殖以及生存能力和重塑环境能力选择的进化事件。进化记忆在StPM - 加德纳菌生物膜的结构分化中得以巩固，使其能够抵御既往和新出现的挑战。
