Yuan Li, Shang Lei-peng, Liao Qin-xiang, Gui Juan, Zhang Lin
West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2013 Dec;30(6):739-41. doi: 10.3760/cma.j.issn.1003-9406.2013.06.024.
To investigate genetic polymorphisms of 10 short tandem repeats loci (D6S1043, D7S3048, D9S925, D10S2325, D11S2368, D14S608, D15S659, D17S1290, D20S470 and GATA198B05) in Tibetans from Lhasa, China.
Following extraction, DNA from 208 unrelated Tibetan individuals was amplified with a self-designed multiplex PCR system. The amplified fragments were separated by electrophoresis on an ABI 3130 Genetic Analyzer and analyzed with GeneMapper®3.2 software.
The distributions of genotype for the 10 STR loci in the population were in accordance with Hardy-Weinberg equilibrium. The polymorphism information component for the 10 loci was 0.750-0.860, the degree of heterozygosity was 0.726-0.865, the discrimination power was 0.919-0.968, and the probability of exclusion was 0.470-0.725. The combined probability of exclusion and combined discrimination power was 0.9998 and 0.999 999 999 997, respectively.
Above STR loci have high probability of exclusion and discrimination power, which can be used as candidate markers for population genetic research and forensic practice for Tibetans from Lhasa, China.
研究中国拉萨藏族人群中10个短串联重复序列位点（D6S1043、D7S3048、D9S925、D10S2325、D11S2368、D14S608、D15S659、D17S1290、D20S470和GATA198B05）的基因多态性。
提取208名无血缘关系的藏族个体的DNA后，用自行设计的多重聚合酶链反应（PCR）系统进行扩增。扩增片段在ABI 3130基因分析仪上进行电泳分离，并用GeneMapper®3.2软件进行分析。
该人群中10个STR位点的基因型分布符合哈迪-温伯格平衡。10个位点的多态性信息含量为0.750 - 0.860，杂合度为0.726 - 0.865，鉴别力为0.919 - 0.968，排除概率为0.470 - 0.725。排除概率总和与鉴别力总和分别为0.9998和0.999999999997。
上述STR位点具有较高的排除概率和鉴别力，可作为中国拉萨藏族人群群体遗传学研究和法医学实践的候选标记。
