Li Peiyao, Yan Hongbo, Zhang Hongxing, Yu Lixia, Wang Zhifu, Zhai Yun, Xia Xia, Zhang Jiye, Zhang Yang, Ma Fuchao, Huang Wenfeng, Cai Mi, Cui Ying, He Fuchu, Zhou Gangqiao
1 State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine , Beijing, People's Republic of China .
Genet Test Mol Biomarkers. 2014 Mar;18(3):149-55. doi: 10.1089/gtmb.2013.0400. Epub 2013 Dec 13.
Genetic variations in microRNAs may alter their processing, expression, and binding to target mRNAs, consequently affecting many cancer-related biological processes. Recently, a polymorphism rs11614913 in MIR196A2 was shown to affect the processing of the precursor microRNA into its mature forms and the repertoire of target mRNAs with which it interacts. We examined whether this polymorphism was relevant to the risk of occurrence or progression of nasopharyngeal carcinoma (NPC) in the Chinese population.
We genotyped the MIR196A2 rs11614913 in a case-control study of 1084 patients with NPC and 1036 cancer-free controls using the TaqMan assay. The genetic associations with the risk of occurrence and progression of NPC were analyzed by logistic regression.
We observed a significantly increased occurrence of NPC associated with the rs11614913 T allele (odds ratio [OR]=1.15, 95% confidence interval [CI]=1.02-1.32, p=0.026) compared with the C allele. The T allele was also significantly associated with the advanced local tumor invasion (T₃+T₄ vs. T₁+T₂; OR=1.27, 95% CI=1.04-1.54, p=0.015) and advanced lymph node metastasis (N₂+N₃ vs. N₀+N₁; OR=1.23, 95% CI=1.02-1.49, p=0.031) of NPC compared with the C allele. Furthermore, stratified analysis indicated that the increased susceptibility to advanced lymph node metastasis of NPC related to the T allele was more pronounced in patients with a positive family history (N₂+N₃ vs. N₀+N₁; p=0.016, test for homogeneity).
Our study suggests that the functional polymorphism rs11614913 in the MIR196A2 gene may contribute to the risk of occurrence and progression of NPC in the Chinese population.
微小RNA中的基因变异可能会改变其加工、表达以及与靶mRNA的结合，从而影响许多与癌症相关的生物学过程。最近，有研究表明MIR196A2基因中的一个多态性位点rs11614913会影响前体微小RNA加工成成熟形式的过程以及与之相互作用的靶mRNA的种类。我们研究了该多态性是否与中国人群鼻咽癌（NPC）的发生或进展风险相关。
我们采用TaqMan分析法，在一项包含1084例NPC患者和1036例无癌对照的病例对照研究中，对MIR196A2基因的rs11614913进行基因分型。通过逻辑回归分析该基因与NPC发生和进展风险的相关性。
与C等位基因相比，我们观察到rs11614913的T等位基因与NPC的发生显著增加相关（比值比[OR]=1.15，95%置信区间[CI]=1.02-1.32，p=0.026）。与C等位基因相比，T等位基因还与NPC的局部肿瘤晚期侵袭（T₃+T₄ 对比 T₁+T₂；OR=1.27，95% CI=1.04-1.54，p=0.015）和晚期淋巴结转移（N₂+N₃ 对比 N₀+N₁；OR=1.23，95% CI=1.02-1.49，p=0.031）显著相关。此外，分层分析表明，T等位基因相关的NPC晚期淋巴结转移易感性增加在有家族史的患者中更为明显（N₂+N₃ 对比 N₀+N₁；p=0.016，齐性检验）。
我们的研究表明，MIR196A2基因中功能性多态性位点rs11614913可能与中国人群NPC的发生和进展风险相关。
