双层极化对离子传输的影响。
Effects of double-layer polarization on ion transport.
作者信息
Hainsworth A H, Hladky S B
出版信息
Biophys J. 1987 Jan;51(1):27-36. doi: 10.1016/S0006-3495(87)83308-8.
Abstract
It has been proposed that changes in ionic strength will alter the shape of current-voltage relations for ion transport across a lipid membrane. To investigate this effect, we measured currents across glyceryl monooleate membranes at applied potentials between 10 and 300 mV using either gramicidin and 1 mM NaCl or valinomycin and 1 mM KCl. A bridge circuit with an integrator as null detector was used to separate the capacitative and ionic components of the current. The changes in the current-voltage relations when ionic strength is varied between 1 and 100 mM are compared with predictions of Gouy-Chapman theory for the effects of these variations on polarization of the electrical diffuse double-layer. Double-layer polarization accounts adequately for the changes observed using membranes made permeable by either gramicidin or valinomycin.
摘要
有人提出，离子强度的变化会改变离子跨脂质膜运输的电流-电压关系的形状。为了研究这种效应，我们使用短杆菌肽和1 mM NaCl或缬氨霉素和1 mM KCl，在10至300 mV的外加电位下测量了单油酸甘油酯膜上的电流。使用带有积分器作为零探测器的桥路来分离电流的电容性和离子性成分。将离子强度在1至100 mM之间变化时电流-电压关系的变化与古依-查普曼理论对这些变化对电扩散双层极化影响的预测进行了比较。双层极化充分解释了使用短杆菌肽或缬氨霉素使膜具有通透性时观察到的变化。
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本文引用的文献
1
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2
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J Physiol. 1952 Apr;116(4):449-72. doi: 10.1113/jphysiol.1952.sp004717.
3
Electrostatic modeling of ion pores. Energy barriers and electric field profiles.离子通道的静电建模。能垒与电场分布
Biophys J. 1982 Aug;39(2):157-64. doi: 10.1016/S0006-3495(82)84503-7.
4
Ion currents through pores. The roles of diffusion and external access steps in determining the currents through narrow pores.通过孔道的离子电流。扩散和外部通道步骤在决定通过狭窄孔道的电流中的作用。
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