Department of Normal Pathological and Clinical Physiology, Prague, Czech Republic.
Physiol Res. 2013;62(Suppl 1):S67-74. doi: 10.33549/physiolres.932576.
An epileptic seizure and postictal period in addition to well-known features are also characterized by massive consumption of energy. This is thought to lead to oxidative stress and increased generation of free radicals, which is reflected by increased levels of oxidative products. Our previous work described the neuroprotective effects of melatonin in preventing cognitive worsening after a single epileptic seizure. This work was aimed on direct measurement of free radicals in brain tissue using the EPR method 1, 15 and 60 minutes after seizure. The measurement was performed in adult male Wistar rats at the mentioned intervals after a single tonic-clonic seizure induced by flurothyl. In comparison to control animals there was a significant increase in hydroxyl and nitroxyl radicals 60 minutes after the seizure. The levels of hydroxyl radicals were significantly lower in animals that received melatonin 60 minutes before seizure induction compared to animals without preventive treatment. Therefore, melatonin affected the generation of the measured free radicals differently. An important finding was the delayed increase in free radicals after a single seizure in the later phases of recovery.
癫痫发作和发作后时期除了众所周知的特征外，还表现为能量的大量消耗。这被认为会导致氧化应激和自由基的产生增加，这反映在氧化产物水平的增加上。我们之前的工作描述了褪黑素在预防单次癫痫发作后认知恶化方面的神经保护作用。这项工作旨在使用 EPR 方法直接测量脑组织中的自由基，分别在癫痫发作后 1、15 和 60 分钟进行测量。在氟烷诱导的单次强直-阵挛性癫痫发作后，在成年雄性 Wistar 大鼠的上述时间间隔内进行了测量。与对照组动物相比，在癫痫发作后 60 分钟时，羟自由基和氮氧自由基的水平显著增加。与没有预防性治疗的动物相比，在癫痫发作前 60 分钟给予褪黑素的动物的羟自由基水平显著降低。因此，褪黑素对所测量的自由基的产生有不同的影响。一个重要的发现是在恢复的后期阶段，单次癫痫发作后自由基的延迟增加。
