Department Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands.
FEMS Microbiol Ecol. 2014 Mar;87(3):639-49. doi: 10.1111/1574-6941.12252. Epub 2013 Dec 12.
It is increasingly recognized that volatile organic compounds play an import role during interactions between soil microorganisms. Here, we examined the possible involvement of volatiles in the interaction of Collimonas bacteria with soil fungi. The genus Collimonas is known for its ability to grow at the expense of living fungi (mycophagy), and antifungal volatiles may contribute to the attack of fungi by these bacteria. We analyzed the composition of volatiles produced by Collimonas on agar under different nutrient conditions and studied the effect on fungal growth. The volatiles had a negative effect on the growth of a broad spectrum of fungal species. Collimonas bacteria did also produce volatiles in sand microcosms supplied with artificial root exudates. The production of volatiles in sand microcosms was enhanced by the presence of fungi. The overall picture that we get from our study is that antifungal volatiles produced by Collimonas could play an important role in realizing its mycophagous lifestyle. The current work is also interesting for understanding the ecological relevance of volatile production by soil bacteria in general as we found strong influences of root exudates composition and incubation conditions on the spectrum of volatiles produced.
越来越多的人认识到，挥发性有机化合物在土壤微生物相互作用中起着重要作用。在这里，我们研究了挥发性物质在 Collimonas 细菌与土壤真菌相互作用中的可能作用。已知 Collimonas 属能够以活真菌（真菌食性）为代价生长，并且抗真菌挥发性物质可能有助于这些细菌攻击真菌。我们分析了 Collimonas 在不同营养条件下琼脂上产生的挥发性物质的组成，并研究了其对真菌生长的影响。这些挥发性物质对广谱真菌物种的生长有负面影响。Collimonas 细菌在含有人工根分泌物的沙微宇宙中也产生挥发性物质。真菌的存在增强了沙微宇宙中挥发性物质的产生。我们从研究中得到的总体情况是，Collimonas 产生的抗真菌挥发性物质可能在实现其真菌食性生活方式中发挥重要作用。目前的工作对于理解土壤细菌产生挥发性物质的生态相关性也很有趣，因为我们发现根分泌物组成和培养条件对产生的挥发性物质谱有很强的影响。
