RNA菌落杂交法。
RNA colony hybridization method.
作者信息
Ivanov I, Gigova L
出版信息
Gene. 1986;46(2-3):287-90. doi: 10.1016/0378-1119(86)90413-0.
PMID:2433191
Abstract
A method for rapid screening of specific RNA sequences in recombinant colonies by hybridization in situ is presented. The method includes two consecutive steps of lytic treatment of the nitrocellulose-filter-supported colonies (10% sodium dodecyl sulfate and 3 X SSC at 65 degrees C) and hybridization with 32P-labelled specific oligodeoxynucleotides.
摘要
本文介绍了一种通过原位杂交快速筛选重组菌落中特定RNA序列的方法。该方法包括对硝酸纤维素滤膜支持的菌落进行裂解处理（在65℃下用10%十二烷基硫酸钠和3X SSC）以及与32P标记的特定寡脱氧核苷酸杂交这两个连续步骤。
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