Zoufan Keivan, Jiang Jin, Komabayashi Takashi, Wang Yu-Hsiung, Safavi Kamran E, Zhu Qiang
Division of Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030-1715, USA.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Nov;112(5):657-61. doi: 10.1016/j.tripleo.2011.03.050. Epub 2011 Jul 20.
This study evaluated the cytotoxicity of GuttaFlow and EndoSequence BC sealers and compared them with AH Plus and Tubli-Seal sealers.
Samples (0.5 mg) of freshly mixed or set BC, GuttaFlow, AH Plus, and Tubli-Seal sealers were eluted with 300, 600, and 1,000 μL cell culture medium for 24 and 72 hours. L929 cells were seeded into 96-well plates at 3 × 10(4) cells/well and cultured with 100 μL eluate from each eluate group. Cells cultured only with culture medium served as control. After 24 hours' incubation, the cytotoxicity was evaluated by MTT assay. Cell viability was calculated as the percentage of the control group, and the results were analyzed with a one-way analysis of variance.
For the freshly mixed sealer, cell viability in the AH Plus group was less than in all of the other 3 sealer groups. The Tubli-Seal sealer group had less cell viability than the EndoSequence BC and GuttaFlow sealer groups. For the set sealer, the Tubli-Seal and AH Plus groups had less cell viability than the EndoSequence BC and GuttaFlow sealer groups. There was no cell viability difference between the EndoSequence BC and GuttaFlow sealer groups in the either freshly mixed or set sealer group.
The GuttaFlow and EndoSequence BC sealers have lower cytotoxicity than the AH Plus and Tubli-Seal sealers.
本研究评估了GuttaFlow和EndoSequence BC封闭剂的细胞毒性，并将它们与AH Plus和Tubli-Seal封闭剂进行比较。
将新鲜混合或凝固后的BC、GuttaFlow、AH Plus和Tubli-Seal封闭剂的样品（0.5毫克）分别用300、600和1000微升细胞培养基洗脱24小时和72小时。将L929细胞以3×10⁴个细胞/孔接种到96孔板中，并用来自每个洗脱组的100微升洗脱液进行培养。仅用培养基培养的细胞作为对照。孵育24小时后，通过MTT法评估细胞毒性。细胞活力以对照组的百分比计算，并采用单因素方差分析对结果进行分析。
对于新鲜混合的封闭剂，AH Plus组的细胞活力低于其他3种封闭剂组。Tubli-Seal封闭剂组的细胞活力低于EndoSequence BC和GuttaFlow封闭剂组。对于凝固后的封闭剂，Tubli-Seal和AH Plus组的细胞活力低于EndoSequence BC和GuttaFlow封闭剂组。在新鲜混合或凝固后的封闭剂组中，EndoSequence BC和GuttaFlow封闭剂组之间的细胞活力没有差异。
GuttaFlow和EndoSequence BC封闭剂的细胞毒性低于AH Plus和Tubli-Seal封闭剂。
