Broers J L, Carney D N, Klein Rot M, Schaart G, Lane E B, Vooijs G P, Ramaekers F C
J Cell Sci. 1986 Jul;83:37-60. doi: 10.1242/jcs.83.1.37.
The intermediate filament protein (IFP) characteristics of a panel of lung cancer cell lines including adenocarcinoma (two cell lines) and small cell lung cancer (SCLC, three classic and three variant cell lines) were examined using one- and two-dimensional gel electrophoretic techniques, immunocytochemical techniques and immunoblotting assays. A panel of 28 monoclonal and polyclonal antibodies to the five different types of IFP were used. The results of our studies indicate that these human lung adenocarcinoma, classic SCLC and variant SCLC cell lines can be differentiated on the basis of their pattern of IFP. The main conclusions from this study can be summarized as follows. The two adenocarcinoma cell lines contain cytokeratins 7, 8, 18, and sometimes 19, next to vimentin intermediate filament (IF). The three classic-type SCLC cell lines contain only cytokeratin IFs but not vimentin IF or neurofilaments (NFs). Cytokeratin polypeptides 7, 8, 18 and 19 could be detected. All three variant-type SCLC cell lines do not contain detectable amounts of cytokeratins. In contrast, two out of three variant SCLC cell lines contain neurofilament proteins. All three variant-type SCLC cell lines contain vimentin IF. Using immunoblotting assays with monoclonal and polyclonal antibodies to defined NF proteins the presence of the 68 X 10(3) Mr and the 160 X 10(3) Mr NF polypeptide could be demonstrated in two variant SCLC cell lines. As patients with SCLC-variant phenotype have a poorer prognosis after cytotoxic therapy than patients with 'pure' SCLC, the use of antibodies to IFP in staining fresh lung tumours, especially anaplastic ones, may differentiate the two subtypes of SCLC. Such a distinction would have a major impact on therapy selections and may be of prognostic importance.
运用一维及二维凝胶电泳技术、免疫细胞化学技术和免疫印迹分析，检测了一组肺癌细胞系的中间丝蛋白（IFP）特征，该组细胞系包括腺癌（两个细胞系）和小细胞肺癌（SCLC，三个经典细胞系和三个变异细胞系）。使用了一组针对五种不同类型IFP的28种单克隆和多克隆抗体。我们的研究结果表明，这些人肺腺癌、经典SCLC和变异SCLC细胞系可根据其IFP模式进行区分。本研究的主要结论可总结如下。两个腺癌细胞系除波形蛋白中间丝（IF）外，还含有细胞角蛋白7、8、18，有时还有19。三个经典型SCLC细胞系仅含有细胞角蛋白IF，而不含有波形蛋白IF或神经丝（NF）。可检测到细胞角蛋白多肽7、8、18和19。所有三个变异型SCLC细胞系均未检测到可检测量的细胞角蛋白。相反，三个变异SCLC细胞系中有两个含有神经丝蛋白。所有三个变异型SCLC细胞系均含有波形蛋白IF。使用针对特定NF蛋白的单克隆和多克隆抗体进行免疫印迹分析，在两个变异SCLC细胞系中可证实存在68×10³Mr和160×10³Mr的NF多肽。由于具有SCLC变异表型的患者在细胞毒性治疗后的预后比“纯”SCLC患者差，因此在对新鲜肺肿瘤（尤其是间变性肿瘤）进行染色时使用IFP抗体可能会区分SCLC的两种亚型。这种区分对治疗选择可能会产生重大影响，并且可能具有预后意义。
