Broers J L, Carney D N, de Ley L, Vooijs G P, Ramaekers F C
Proc Natl Acad Sci U S A. 1985 Jul;82(13):4409-13. doi: 10.1073/pnas.82.13.4409.
The expression of intermediate filament proteins in classic and variant-type small-cell lung carcinoma (SCLC) cell lines was studied using immunocytochemical techniques, two-dimensional gel electrophoresis and immunoblotting assays. Classic SCLC cell lines contain cytokeratin proteins but no neurofilaments. In contrast, variant cell lines do not contain detectable amounts of cytokeratins but partly express neurofilaments and vimentin. These results explain apparent discrepancies on the intermediate filament content of SCLC described in the recent literature. The application of antibodies to fresh biopsy specimens of SCLC may in the future allow the identification of the variant type of cells in clinical SCLC specimens and may have a major impact on therapeutic strategy and prognosis in these patients.
运用免疫细胞化学技术、二维凝胶电泳和免疫印迹分析，研究了经典型和变异型小细胞肺癌（SCLC）细胞系中间丝蛋白的表达情况。经典SCLC细胞系含有细胞角蛋白，但不含神经丝。相反，变异细胞系不含可检测量的细胞角蛋白，但部分表达神经丝和波形蛋白。这些结果解释了近期文献中描述的SCLC中间丝含量明显存在的差异。未来，将抗体应用于SCLC新鲜活检标本，可能有助于识别临床SCLC标本中的变异细胞类型，并可能对这些患者的治疗策略和预后产生重大影响。
