Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutics Science, Federal University of Piauí, 64.049-550 Teresina, Piauí, Brazil.
Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutics Science, Federal University of Piauí, 64.049-550 Teresina, Piauí, Brazil; Department of Pharmacy, Southern University Bangladesh, 22-Shahid Mirza Lane (E), Academic building-II, 739/A, Mehedibag Road, Mehedibag-4000, Chittagong, Bangladesh.
Brain Res. 2014 Feb 14;1547:34-42. doi: 10.1016/j.brainres.2013.12.003. Epub 2013 Dec 10.
Phytol, a branched chain unsaturated alcohol, is particularly interesting because it is an isolated compound from essential oils of different medicinal plants. The aim of this study was to evaluate the anxiolytic-like effects of phytol in animal models to clarify their possible action mechanism. After acute intraperitoneal treatment with phytol at doses of 25, 50 and 75 mg/kg behavioral models of open-field, elevated-plus-maze, rota-rod, light-dark, marble-burying and pentobarbital sleeping time tests were utilized. In open field test, phytol (25, 50 and 75 mg/kg) [p<0.01] increased the number of crossings and rearings. However, the number of groomings [p<0.01] was reduced. Likewise, the number of entries and the time spent in light space were increased [p<0.01] while the number of marble-burying was decreased [p<0.001], in elevated-plus-maze, light-dark and marble-burying tests, respectively. In motor activity test, phytol (75 mg/kg) impaired the rota-rod performance of mice [p<0.01]. In pentobarbital sleeping time test, phytol 75 mg/kg decreased for latency of sleeping and phytol (25, 50 and 75 mg/kg) increased the sleep time when compared to negative control [p<0.05]. All these effects were reversed by pre-treatment with flumazenil (2.5mg/kg, i.p.), similarly to those observed with diazepam (2mg/kg, i.p.; positive control) suggesting that the phytol presents mechanism of action by interaction with the GABAergic system. These findings suggest that acute administration of phytol exerts an anxiolytic-like effect on mice. Furthermore, suppose that phytol interacts with GABAA receptor, probably at the receptor subtypes that mediate benzodiazepines effects, to produce sedative and anxiolytic activities.
植物醇是一种支链不饱和醇，特别有趣，因为它是从不同药用植物的精油中分离出来的化合物。本研究旨在评估植物醇在动物模型中的抗焦虑样作用，以阐明其可能的作用机制。急性腹腔内给予植物醇 25、50 和 75mg/kg 后，利用旷场、高架十字迷宫、转棒、明暗箱、藏珠和戊巴比妥睡眠时间试验进行行为模型测试。在旷场试验中，植物醇（25、50 和 75mg/kg）[p<0.01]增加了穿越和后肢的次数。然而，梳理次数减少[p<0.01]。同样，进入次数和在亮区的时间增加[p<0.01]，而在高架十字迷宫、明暗箱和藏珠试验中，埋珠次数减少[p<0.001]。在运动活动试验中，植物醇（75mg/kg）损害了小鼠的转棒性能[p<0.01]。在戊巴比妥睡眠时间试验中，与阴性对照相比，植物醇 75mg/kg 降低了睡眠潜伏期，植物醇（25、50 和 75mg/kg）增加了睡眠时间[p<0.05]。这些作用都被氟马西尼（2.5mg/kg，腹腔内）预处理所逆转，与地西泮（2mg/kg，腹腔内；阳性对照）观察到的作用相似，表明植物醇通过与 GABA 能系统相互作用呈现作用机制。这些发现表明，急性给予植物醇对小鼠产生抗焦虑样作用。此外，假设植物醇与 GABAA 受体相互作用，可能作用于介导苯二氮䓬类效应的受体亚型，产生镇静和抗焦虑作用。
