College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China.
Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Anhui Agricultural University, Hefei, P.R. China.
PLoS One. 2018 Sep 25;13(9):e0204210. doi: 10.1371/journal.pone.0204210. eCollection 2018.
Gut microbial ecology is responsible for fatty acid metabolism in ruminants. The cecal microbiota composition of geese and their adaptation to fiber inclusion and feeding timeswere investigated in this study. A total of 116 Wanxi white geese were randomly selected at 70 days old. Eight geese were subjected to cecal sampling at 70 d of age, and the remaining 108 geese were divided into four groups with three replicates each (9 geese in each replicate). The geese in the four groups were fed 0, 15, 30, and 45% green forage (relative to dry matter), respectively. Three birds from each replicate were selected for cecal sampling at 80, 90, and 100 days old. All samples were subjected to 16S rRNA gene sequencing using the Illumina Ion Personal Genome Machine platform. Bacterial abundance was analyzed using two-way ANOVA analysis, and the relationship between the relative abundance of bacteria (phylum level) and fatty acids was analyzed using acanonical correspondence analysis. Cecal microbiota in geese were mainly composed of Bacteroidetes (68.46%), Firmicutes (20.04%), and Proteobacteria (7.89%). Dietary treatments had no significant effect on the α-diversity indices of the cecal bacterial community (P > 0.05), but a numerical increase occurred with increased fattening duration and green forage inclusion. The Selenomonadales order (P = 0.024), Negativicutes class (P = 0.026), and Megamonas (P = 0.012) and Oscillospira (P = 0.042) genera were affected by green forageinclusion level, and microflora abundance was mainly influenced by the fattening duration. Bacteria phyla were mostly set along the line of linolenic acid and oleic acid. Finally, Bacteroidales might be an intestinal promoter that improves unsaturated fatty acid synthesis in geese.
肠道微生物生态学负责反刍动物的脂肪酸代谢。本研究调查了鹅的盲肠微生物组成及其对纤维含量和喂养时间的适应能力。总共随机选择了 116 只皖西白鹅，在 70 日龄时进行了 8 只鹅的盲肠采样，其余 108 只鹅分为 4 组，每组 3 个重复（每个重复 9 只）。4 组鹅分别饲喂 0%、15%、30%和 45%的青绿饲料（相对于干物质）。每组选择 3 只进行盲肠采样，采样时间分别为 80、90 和 100 日龄。所有样品均采用 Illumina Ion Personal Genome Machine 平台进行 16S rRNA 基因测序。使用双向方差分析分析细菌丰度，使用典范对应分析（CCA）分析细菌相对丰度（门水平）与脂肪酸之间的关系。鹅的盲肠微生物主要由拟杆菌门（68.46%）、厚壁菌门（20.04%）和变形菌门（7.89%）组成。日粮处理对盲肠细菌群落的α多样性指数没有显著影响（P > 0.05），但随着育肥时间和青绿饲料添加量的增加而出现数值增加。塞尔诺蒙纳德斯目（P = 0.024）、Negativicutes 纲（P = 0.026）和 Megamonas（P = 0.012）和 Oscillospira（P = 0.042）属受青绿饲料添加水平的影响，微生物丰度主要受育肥时间的影响。细菌门主要沿着亚麻酸和油酸的线设置。最后，拟杆菌门可能是一种肠道促进剂，可提高鹅不饱和脂肪酸的合成。
