老年和年轻工蜂（Apis mellifera）细胞降解活性的变化。
Changes in cellular degradation activity in young and old worker honeybees (Apis mellifera).
机构信息
Department of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
出版信息
Exp Gerontol. 2014 Feb;50:128-36. doi: 10.1016/j.exger.2013.12.003. Epub 2013 Dec 14.
The trophocytes and fat cells of honeybees (Apis mellifera) have been used in cellular senescence studies, but the changes of cellular degradation activity with aging in workers are unknown. In this study, cellular degradation activity was evaluated in the trophocytes and fat cells of young and old workers reared in a field hive. The results showed the following: (1) 20S proteosome activity decreased with aging, whereas its expression increased with aging; (2) the expression of microtubule-associated protein 1 light chain 3-II (LC3-II) and the 70 kD heat shock cognate protein (Hsc70) decreased with aging; (3) the size and number of autophagic vacuoles decreased with aging; (4) p62/SQSTM1 and polyubiquitin aggregate expression decreased with aging; (5) lysosomal efficiency decreased with aging; and (6) molecular target of rapamycin (mTOR) expression increased with aging. These results indicate that young workers have higher levels of cellular degradation activity than old workers and that aging results in a decline in the cellular degradation activity in worker honeybees.
蜜蜂（Apis mellifera）的滋养细胞和脂肪细胞已被用于细胞衰老研究，但工蜂衰老过程中细胞降解活性的变化尚不清楚。在这项研究中，评估了在野外蜂群中饲养的年轻和年老工蜂的滋养细胞和脂肪细胞中的细胞降解活性。结果表明：（1）20S 蛋白酶体活性随衰老而降低，但其表达随衰老而增加；（2）微管相关蛋白 1 轻链 3-II（LC3-II）和 70kD 热休克同源蛋白（Hsc70）的表达随衰老而降低；（3）自噬小体的大小和数量随衰老而减少；（4）p62/SQSTM1 和多聚泛素聚合体的表达随衰老而减少；（5）溶酶体效率随衰老而降低；（6）雷帕霉素（mTOR）的表达随衰老而增加。这些结果表明，年轻的工蜂比年老的工蜂具有更高水平的细胞降解活性，而衰老会导致工蜂细胞降解活性下降。
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