Silva-Frade Camila, Gameiro Roberto, Okamura Lucas Hidenori, Flores Eduardo Furtado, Cardoso Tereza Cristina
UNESP-São Paulo State University, Laboratory of Animal Virology and Cell Culture, College of Veterinary Medicine, São Paulo 16050-680, Brazil.
UNESP-São Paulo State University, Departament of Preventive Veterinary Medicine, Federal University of Santa Maria, UFSM, College of Veterinary Medicine, Santa Maria, RS 97115-900, Brazil.
Mol Cell Probes. 2014 Aug;28(4):113-7. doi: 10.1016/j.mcp.2013.10.005. Epub 2013 Dec 13.
In vitro-produced bovine embryos become infected after exposure to bovine Herpesvirus type 5 (BoHV-5), yet no changes in developmental rates, mitochondrial activity and inhibition of apoptosis are detected in comparison to unexposed embryos. Thus, the aim of the present study was to assess the transcription of mitochondria-mediated apoptosis genes using TaqMan real-time polymerase chain reaction. Transcripts of mcl-1, caspase-2, -3, Apaf-1 and Bax genes were measured after exposure to BoHV-5 in vitro. Mitochondrial dehydrogenase activity was evaluated by MTT test and compared between groups of exposed and unexposed embryos, at day 7 of development. The rate of oocyte maturation was assessed by the extrusion of the first polar body. In summary, BoHV-5 exposed embryos retained their viability, mitochondrial dehydrogenase activity and displayed up-regulation of transcription of survival mcl-1 gene and down-regulation of Bax transcription in relation to mitochondria-mediated pathway which might improve embryo viability. These findings demonstrate that BoHV-5 exposed embryos maintain their viability and mitochondrial dehydrogenase activity with no compromise of embryos produced in vitro.
