Laboratorio de Sinovioanálisis Molecular, Instituto Nacional de Rehabilitación, Calz. México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P 14389, D.F., México.
Food Funct. 2014 Feb;5(2):303-9. doi: 10.1039/c3fo60135h.
Cellular oxidative stress produced by an increase in free radicals is one of the factors that promote the development of chronic degenerative diseases; therefore, consuming natural antioxidants helps minimize their negative effects. This study evaluated the cytotoxicity of the soursop extract (Annona muricata), its cytoprotective capacity against oxidative stress induced by hydrogen peroxide, the inhibitory potential of reactive oxygen species (ROS), the molecular mechanism of its antioxidant action, and its capacity to repair cellular damage in the fibroblast cell line. The soursop extract proved not to be cytotoxic in fibroblast cultures and showed cytoprotective capacity against hydrogen peroxide-induced stress; in cell culture it reduced the generation of ROS significantly by inhibiting a sub-unit of the NADPH oxidase enzyme (p47phox). The soursop extract can prevent damage caused by cellular oxidants.
细胞内自由基增加导致的氧化应激是促进慢性退行性疾病发展的因素之一；因此，摄入天然抗氧化剂有助于最大限度地减少其负面影响。本研究评估了刺果番荔枝提取物（Annona muricata）的细胞毒性、其对过氧化氢诱导的氧化应激的细胞保护能力、活性氧（ROS）的抑制潜力、其抗氧化作用的分子机制以及修复成纤维细胞系细胞损伤的能力。刺果番荔枝提取物在成纤维细胞培养中被证明没有细胞毒性，并显示出对过氧化氢诱导的应激的细胞保护能力；在细胞培养中，它通过抑制 NADPH 氧化酶亚基（p47phox）显著减少 ROS 的生成。刺果番荔枝提取物可以预防细胞氧化剂引起的损伤。
