通过大蒜和彗星试验检测氧化铟锡的遗传毒性。
Genotoxicity of indium tin oxide by Allium and Comet tests.
作者信息
Ciğerci İbrahim Hakkı, Liman Recep, Özgül Emre, Konuk Muhsin
机构信息
Biology Department, Faculty of Science and Literatures, Afyon Kocatepe University, 03200, Afyon, Turkey.
出版信息
Cytotechnology. 2015 Jan;67(1):157-63. doi: 10.1007/s10616-013-9673-0. Epub 2013 Dec 12.
Abstract
Genotoxic effects of indium tin oxide (ITO) were investigated on root cells of Allium cepa by employing both Allium and Comet assays. A. cepa roots were treated with the aqueous dispersions of ITO at 5 different concentrations (12.5, 25, 50, 75, and 100 ppm) for 4 h. Exposure of ITO significantly increased mitotic index, and total chromosomal aberrations by the Allium test. While chromosome laggards, stickiness, disturbed anaphase-telophase and anaphase bridges were observed in anaphase-telophase cells, c-metaphase and binuclear cells were observed in other cells. A significant increase in DNA damage was also observed at all concentrations of ITO by the Comet assay. These results indicate that ITO exhibits genotoxic activity in A. cepa root meristematic cells.
摘要
通过采用洋葱试验和彗星试验，研究了氧化铟锡（ITO）对洋葱根细胞的遗传毒性作用。将洋葱根用5种不同浓度（12.5、25、50、75和100 ppm）的ITO水分散体处理4小时。通过洋葱试验，ITO暴露显著增加了有丝分裂指数和总染色体畸变。在后期-末期细胞中观察到染色体落后、粘连、后期-末期紊乱和后期桥，在其他细胞中观察到c-中期和双核细胞。通过彗星试验，在所有浓度的ITO下也观察到DNA损伤显著增加。这些结果表明，ITO在洋葱根分生细胞中表现出遗传毒性活性。
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