Liman Recep, Başbuğ Bermal, Ali Muhammad Muddassir, Acikbas Yaser, Ciğerci İbrahim Hakkı
Faculty of Arts and Sciences, Molecular Biology and Genetics Department, Usak University, 64300, Uşak, Turkey.
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Syed Abdul Qadir Jilani Road (Outfall Road), 54000, Lahore, Pakistan.
J Appl Genet. 2021 Feb;62(1):85-92. doi: 10.1007/s13353-020-00608-x. Epub 2021 Jan 6.
Tungsten oxide nanoparticles or nanopowder (WONPs) is commonly used in various industries and also in biomedical applications such as additives, pigments, and biomedical sensors. Non-judicious excessive use of these nanoparticles (NPs) could be a serious human health concern. Therefore, the current study aimed to explore the cytotoxic and genotoxic assessment of WONPs through Allium cepa anaphase-telophase and comet assays. Nanoparticles were characterized through the scanning and transmission electron microscopy (TEM), zetasizer, and energy-dispersive X-ray spectroscopy. The mean size and the average diameter of WONPs were determined as 21.57 ± 2.48 nm and 349.42 ± 80.65 nm using TEM and a Zetasizer measurement system, respectively. Five concentrations (12.5 mg/L, 25 mg/L, 50 mg/L, 75 mg/L, and 100 mg/L) of WONPs were employed on the Allium cepa (A. cepa) roots for 4 h. Significant (p ≤ 0.05) decrease in mitotic index (MI) was shown by WONPs at all concentrations. The increase of chromosomal aberrations (CAs) was also observed in a concentration-dependent manner due to the WONPs exposure. There was a significant increase (p ≤ 0.05) in DNA damage at all concentrations of WONPs on the A. cepa cells. It was concluded that WONPs had cytotoxic and genotoxic effects on A. cepa meristematic cells. Moreover, further cytogenetic effects of WONPs should be investigated at the molecular level to assess its safety margin.
氧化钨纳米颗粒或纳米粉末（WONPs）常用于各种行业，也用于生物医学应用，如添加剂、颜料和生物医学传感器。不合理地过度使用这些纳米颗粒（NPs）可能会严重影响人类健康。因此，本研究旨在通过洋葱根尖后期 - 末期和彗星试验来探索WONPs的细胞毒性和遗传毒性评估。通过扫描电子显微镜、透射电子显微镜（TEM）、zeta电位仪和能量色散X射线光谱对纳米颗粒进行表征。使用TEM和Zetasizer测量系统分别测定WONPs的平均尺寸和平均直径为21.57±2.48纳米和349.42±80.65纳米。将五种浓度（12.5毫克/升、25毫克/升、50毫克/升、75毫克/升和100毫克/升）的WONPs作用于洋葱（A. cepa）根尖4小时。所有浓度的WONPs均显示有丝分裂指数（MI）显著（p≤0.05）下降。由于暴露于WONPs，还观察到染色体畸变（CAs）以浓度依赖性方式增加。在所有浓度的WONPs作用下，洋葱细胞的DNA损伤均显著增加（p≤0.05）。得出的结论是，WONPs对洋葱分生组织细胞具有细胞毒性和遗传毒性。此外，应在分子水平上进一步研究WONPs的细胞遗传学效应，以评估其安全边际。
