Horiguchi J, Warren M K, Ralph P, Kufe D
Biochem Biophys Res Commun. 1986 Dec 30;141(3):924-30. doi: 10.1016/s0006-291x(86)80131-0.
We and others have previously demonstrated expression of the co-fms proto-oncogene during human monocytic differentiation. The c-fms gene has since been shown to encode for the macrophage specific colony stimulating factor (CSF-1) receptor. The present results demonstrate that both CSF-1 and c-fms transcripts are induced during monocytic differentiation of human HL-60 leukemia cells. The results further demonstrate that normal human monocytes express CSF-1 RNA and that the level of these transcripts increases upon treatment with phorbol ester. Finally, the detection of CSF-1 RNA in HL-60 cells and in monocytes is associated with production of the CSF-1 gene product. These findings would suggest that monocytes are capable of regulating their own survival, growth and differentiation through CSF-1 production.
我们和其他研究人员先前已证明原癌基因c-fms在人类单核细胞分化过程中表达。此后，c-fms基因已被证明编码巨噬细胞特异性集落刺激因子（CSF-1）受体。目前的结果表明，在人类HL-60白血病细胞的单核细胞分化过程中，CSF-1和c-fms转录本均被诱导。结果还进一步表明，正常人类单核细胞表达CSF-1 RNA，并且在用佛波酯处理后这些转录本的水平会增加。最后，在HL-60细胞和单核细胞中检测到CSF-1 RNA与CSF-1基因产物的产生相关。这些发现表明，单核细胞能够通过产生CSF-1来调节自身的存活、生长和分化。
