2011年至2012年中国南方地区鸡贫血病毒的系统发育和分子特征分析

Zhang Xinheng, Liu Yuanjia, Wu Boliang, Sun Baoli, Chen Feng, Ji Jun, Ma Jingyun, Xie Qingmei

College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China.

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

Sci Rep. 2013 Dec 17;3:3519. doi: 10.1038/srep03519.

Chicken anemia virus (CAV) is an important pathogen that causes severe immunosuppression in young chickens. We have characterized 13 CAVs isolated from different commercial farms in southern China between 2011 and 2012. We discovered 92 variable residues compared to 37 other CAV complete genome sequences from other parts of the world listed in GenBank; these residues have not been previously observed. All of the Chinese CAV genomes that were characterized in this study had a glutamine at position 394, a hallmark of highly pathogenic CAVs. We also discovered that intra-group genetic recombination plays a role in generating genetic diversity in natural populations of CAV. The GD-J-12 isolate was a possible recombinant between GD-C-12 and GD-M-12 in the genomic region that encompassed both the coding and non-coding regions.

鸡贫血病毒（CAV）是一种重要的病原体，可导致雏鸡严重免疫抑制。我们对2011年至2012年间从中国南方不同商业养殖场分离出的13株CAV进行了特征分析。与GenBank中列出的世界其他地区的37个其他CAV完整基因组序列相比，我们发现了92个可变残基；这些残基以前未曾观察到。本研究中鉴定的所有中国CAV基因组在394位都有一个谷氨酰胺，这是高致病性CAV的一个标志。我们还发现，组内基因重组在CAV自然群体的遗传多样性产生中起作用。GD-J-12分离株在包含编码区和非编码区的基因组区域中可能是GD-C-12和GD-M-12之间的重组体。