Dept of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
Dept of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
Exp Eye Res. 2014 Mar;120:50-4. doi: 10.1016/j.exer.2013.12.006. Epub 2013 Dec 15.
Human retinal pigment epithelium cells were used to investigate the mechanisms underlying blood-retinal barrier disruption under conditions of chronic hyperglycemia. The treatment with 25 mM glucose caused a rapid drop in the transepithelial electrical resistance (TEER), which was reversed by the addition of either a methanolic extract from Goji (Lycium barbarum L.) berries or its main component, taurine. Intracellular cAMP levels increased concurrently with the high glucose-induced TEER decrease, and were correlated to an increased activity of the cytosolic isoform of the enzyme adenylyl cyclase. The treatment with plant extract or taurine restored control levels. Data are discussed in view of a possible prevention approach for diabetic retinopathy.
本研究旨在探讨慢性高血糖状态下血视网膜屏障破坏的机制，选用人视网膜色素上皮细胞作为研究对象。结果发现，25mM 葡萄糖处理可导致细胞跨上皮电阻（TEER）迅速下降，而加入枸杞（Lycium barbarum L.）浆果甲醇提取物或其主要成分牛磺酸可逆转这一现象。同时，细胞内环磷酸腺苷（cAMP）水平随着高糖诱导的 TEER 下降而升高，并与胞质型腺苷酸环化酶同工酶活性的增加相关。植物提取物或牛磺酸处理可恢复至对照水平。本文数据提示了一种糖尿病性视网膜病变的可能预防方法。
